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000062893 0247_ $$2DOI$$a10.1002/cbic.200800117
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000062893 084__ $$2WoS$$aBiochemistry & Molecular Biology
000062893 084__ $$2WoS$$aChemistry, Medicinal
000062893 1001_ $$0P:(DE-Juel1)VDB77156$$aThielmann, Y.$$b0$$uFZJ
000062893 245__ $$aAn indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP
000062893 260__ $$aWeinheim$$bWiley-VCH$$c2008
000062893 300__ $$a1767 - 1775
000062893 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000062893 440_0 $$08962$$aChemBioChem$$v9$$x1439-4227
000062893 500__ $$aRecord converted from VDB: 12.11.2012
000062893 520__ $$aThe role of tryptophan as a key residue for ligand binding to the ubiquitin-like modifier GABA(A) receptor associated protein (GABARAP) was investigated. Two tryptophan-binding hydrophobic patches were identified on the conserved face of the GABARAP structure by NMR spectroscopy and molecular docking. GABARAP binding of indole and indole derivatives, including the free amino acid tryptophan was quantified. The two tryptophan binding sites can be clearly distinguished by mapping the NMR spectroscopy-derived residue-specific apparent dissociation constant, K(d), onto the three-dimensional structure of GABARAP. The biological relevance of tryptophan-binding pockets of GABARAP was supported by a highly conserved tryptophan residue in the GABARAP binding region of calreticulin, clathrin heavy chain, and the gamma2 subunit of the GABA(A) receptor. Replacement of tryptophan by alanine abolished ligand binding to GABARAP.
000062893 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000062893 588__ $$aDataset connected to Web of Science, Pubmed
000062893 650_2 $$2MeSH$$aAdaptor Proteins, Signal Transducing: chemistry
000062893 650_2 $$2MeSH$$aAdaptor Proteins, Signal Transducing: metabolism
000062893 650_2 $$2MeSH$$aAmino Acid Sequence
000062893 650_2 $$2MeSH$$aAnimals
000062893 650_2 $$2MeSH$$aBinding Sites
000062893 650_2 $$2MeSH$$aHumans
000062893 650_2 $$2MeSH$$aHydrophobic and Hydrophilic Interactions
000062893 650_2 $$2MeSH$$aIndoles: chemistry
000062893 650_2 $$2MeSH$$aIndoles: metabolism
000062893 650_2 $$2MeSH$$aLigands
000062893 650_2 $$2MeSH$$aMicrotubule-Associated Proteins: chemistry
000062893 650_2 $$2MeSH$$aMicrotubule-Associated Proteins: metabolism
000062893 650_2 $$2MeSH$$aModels, Molecular
000062893 650_2 $$2MeSH$$aMolecular Sequence Data
000062893 650_2 $$2MeSH$$aProtein Conformation
000062893 650_2 $$2MeSH$$aReceptors, GABA-A: metabolism
000062893 650_2 $$2MeSH$$aSubstrate Specificity
000062893 650_2 $$2MeSH$$aTitrimetry
000062893 650_2 $$2MeSH$$aTryptophan: metabolism
000062893 650_7 $$00$$2NLM Chemicals$$aAdaptor Proteins, Signal Transducing
000062893 650_7 $$00$$2NLM Chemicals$$aIndoles
000062893 650_7 $$00$$2NLM Chemicals$$aLigands
000062893 650_7 $$00$$2NLM Chemicals$$aMicrotubule-Associated Proteins
000062893 650_7 $$00$$2NLM Chemicals$$aReceptors, GABA-A
000062893 650_7 $$073-22-3$$2NLM Chemicals$$aTryptophan
000062893 650_7 $$2WoSType$$aJ
000062893 65320 $$2Author$$aGABARAP
000062893 65320 $$2Author$$aindole-binding pocket
000062893 65320 $$2Author$$amolecular modeling
000062893 65320 $$2Author$$amolecular recognition
000062893 65320 $$2Author$$aNMR spectroscopy
000062893 7001_ $$0P:(DE-Juel1)132012$$aMohrlüder, J.$$b1$$uFZJ
000062893 7001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b2$$uFZJ
000062893 7001_ $$0P:(DE-HGF)0$$aStangler, T.$$b3
000062893 7001_ $$0P:(DE-Juel1)VDB57647$$aHartmann, R.$$b4$$uFZJ
000062893 7001_ $$0P:(DE-Juel1)VDB3486$$aBecker, K.$$b5$$uFZJ
000062893 7001_ $$0P:(DE-HGF)0$$aHöltje, H.-D.$$b6
000062893 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b7$$uFZJ
000062893 773__ $$0PERI:(DE-600)2020469-3$$a10.1002/cbic.200800117$$gVol. 9, p. 1767 - 1775$$p1767 - 1775$$q9<1767 - 1775$$tChemBioChem$$v9$$x1439-4227$$y2008
000062893 8567_ $$uhttp://dx.doi.org/10.1002/cbic.200800117
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000062893 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000062893 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
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