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| Hauptseite > Publikationsdatenbank > An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP > print |
| Hauptseite > Publikationsdatenbank > An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP > print |
| 001 | 62893 | ||
| 005 | 20200402210514.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:18567048 |
| 024 | 7 | _ | |2 DOI |a 10.1002/cbic.200800117 |
| 024 | 7 | _ | |2 WOS |a WOS:000258092300014 |
| 037 | _ | _ | |a PreJuSER-62893 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 084 | _ | _ | |2 WoS |a Chemistry, Medicinal |
| 100 | 1 | _ | |a Thielmann, Y. |b 0 |u FZJ |0 P:(DE-Juel1)VDB77156 |
| 245 | _ | _ | |a An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP |
| 260 | _ | _ | |a Weinheim |b Wiley-VCH |c 2008 |
| 300 | _ | _ | |a 1767 - 1775 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a ChemBioChem |x 1439-4227 |0 8962 |v 9 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a The role of tryptophan as a key residue for ligand binding to the ubiquitin-like modifier GABA(A) receptor associated protein (GABARAP) was investigated. Two tryptophan-binding hydrophobic patches were identified on the conserved face of the GABARAP structure by NMR spectroscopy and molecular docking. GABARAP binding of indole and indole derivatives, including the free amino acid tryptophan was quantified. The two tryptophan binding sites can be clearly distinguished by mapping the NMR spectroscopy-derived residue-specific apparent dissociation constant, K(d), onto the three-dimensional structure of GABARAP. The biological relevance of tryptophan-binding pockets of GABARAP was supported by a highly conserved tryptophan residue in the GABARAP binding region of calreticulin, clathrin heavy chain, and the gamma2 subunit of the GABA(A) receptor. Replacement of tryptophan by alanine abolished ligand binding to GABARAP. |
| 536 | _ | _ | |a Funktion und Dysfunktion des Nervensystems |c P33 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK409 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Adaptor Proteins, Signal Transducing: chemistry |
| 650 | _ | 2 | |2 MeSH |a Adaptor Proteins, Signal Transducing: metabolism |
| 650 | _ | 2 | |2 MeSH |a Amino Acid Sequence |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Binding Sites |
| 650 | _ | 2 | |2 MeSH |a Humans |
| 650 | _ | 2 | |2 MeSH |a Hydrophobic and Hydrophilic Interactions |
| 650 | _ | 2 | |2 MeSH |a Indoles: chemistry |
| 650 | _ | 2 | |2 MeSH |a Indoles: metabolism |
| 650 | _ | 2 | |2 MeSH |a Ligands |
| 650 | _ | 2 | |2 MeSH |a Microtubule-Associated Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a Microtubule-Associated Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Models, Molecular |
| 650 | _ | 2 | |2 MeSH |a Molecular Sequence Data |
| 650 | _ | 2 | |2 MeSH |a Protein Conformation |
| 650 | _ | 2 | |2 MeSH |a Receptors, GABA-A: metabolism |
| 650 | _ | 2 | |2 MeSH |a Substrate Specificity |
| 650 | _ | 2 | |2 MeSH |a Titrimetry |
| 650 | _ | 2 | |2 MeSH |a Tryptophan: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Adaptor Proteins, Signal Transducing |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Indoles |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Ligands |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Microtubule-Associated Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Receptors, GABA-A |
| 650 | _ | 7 | |0 73-22-3 |2 NLM Chemicals |a Tryptophan |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a GABARAP |
| 653 | 2 | 0 | |2 Author |a indole-binding pocket |
| 653 | 2 | 0 | |2 Author |a molecular modeling |
| 653 | 2 | 0 | |2 Author |a molecular recognition |
| 653 | 2 | 0 | |2 Author |a NMR spectroscopy |
| 700 | 1 | _ | |a Mohrlüder, J. |b 1 |u FZJ |0 P:(DE-Juel1)132012 |
| 700 | 1 | _ | |a Koenig, B. W. |b 2 |u FZJ |0 P:(DE-Juel1)132009 |
| 700 | 1 | _ | |a Stangler, T. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Hartmann, R. |b 4 |u FZJ |0 P:(DE-Juel1)VDB57647 |
| 700 | 1 | _ | |a Becker, K. |b 5 |u FZJ |0 P:(DE-Juel1)VDB3486 |
| 700 | 1 | _ | |a Höltje, H.-D. |b 6 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Willbold, D. |b 7 |u FZJ |0 P:(DE-Juel1)132029 |
| 773 | _ | _ | |a 10.1002/cbic.200800117 |g Vol. 9, p. 1767 - 1775 |p 1767 - 1775 |q 9<1767 - 1775 |0 PERI:(DE-600)2020469-3 |t ChemBioChem |v 9 |y 2008 |x 1439-4227 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1002/cbic.200800117 |
| 909 | C | O | |o oai:juser.fz-juelich.de:62893 |p VDB |
| 913 | 1 | _ | |k P33 |v Funktion und Dysfunktion des Nervensystems |l Funktion und Dysfunktion des Nervensystems |b Gesundheit |0 G:(DE-Juel1)FUEK409 |x 0 |
| 914 | 1 | _ | |y 2008 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k INB-2 |l Molekulare Biophysik |d 31.12.2008 |g INB |0 I:(DE-Juel1)VDB805 |x 0 |
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| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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