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000062894 0247_ $$2DOI$$a10.1074/jbc.M709655200
000062894 0247_ $$2WOS$$aWOS:000257898800055
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000062894 041__ $$aeng
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000062894 084__ $$2WoS$$aBiochemistry & Molecular Biology
000062894 1001_ $$0P:(DE-HGF)0$$aBailly, A.$$b0
000062894 245__ $$aModulation of P-glycoproteins by auxin transport inhibitors is mediated by interaction with immunophilins
000062894 260__ $$aBethesda, Md.$$bSoc.$$c2008
000062894 300__ $$a21817 - 21826
000062894 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000062894 440_0 $$03091$$aJournal of Biological Chemistry$$v283$$x0021-9258
000062894 500__ $$aRecord converted from VDB: 12.11.2012
000062894 520__ $$aThe immunophilin-like FKBP42 TWISTED DWARF1 (TWD1) has been shown to control plant development via the positive modulation of ABCB/P-glycoprotein (PGP)-mediated transport of the plant hormone auxin. TWD1 functionally interacts with two closely related proteins, ABCB1/PGP1 and ABCB19/PGP19/MDR1, both of which exhibit the ability to bind to and be inhibited by the synthetic auxin transport inhibitor N-1-naphylphtalamic acid (NPA). They are also inhibited by flavonoid compounds, which are suspected modulators of auxin transport. The mechanisms by which flavonoids and NPA interfere with auxin efflux components are unclear. We report here the specific disruption of PGP1-TWD1 interaction by NPA and flavonoids using bioluminescence resonance energy transfer with flavonoids functioning as a classical established inhibitor of mammalian and plant PGPs. Accordingly, TWD1 was shown to mediate modulation of PGP1 efflux activity by these auxin transport inhibitors. NPA bound to both PGP1 and TWD1 but was excluded from the PGP1-TWD1 complex expressed in yeast, suggesting a transient mode of action in planta. As a consequence, auxin fluxes and gravitropism in twd1 roots are less affected by NPA treatment, whereas TWD1 gain-of-function promotes root bending. Our data support a novel model for the mode of drug-mediated P-glycoprotein regulation mediated via protein-protein interaction with immunophilin-like TWD1.
000062894 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000062894 588__ $$aDataset connected to Web of Science, Pubmed
000062894 650_2 $$2MeSH$$aATP-Binding Cassette Transporters: chemistry
000062894 650_2 $$2MeSH$$aArabidopsis: metabolism
000062894 650_2 $$2MeSH$$aArabidopsis Proteins: chemistry
000062894 650_2 $$2MeSH$$aBiological Transport
000062894 650_2 $$2MeSH$$aCell Membrane: metabolism
000062894 650_2 $$2MeSH$$aFlavonoids: chemistry
000062894 650_2 $$2MeSH$$aHomozygote
000062894 650_2 $$2MeSH$$aImmunophilins: chemistry
000062894 650_2 $$2MeSH$$aIndoleacetic Acids: chemistry
000062894 650_2 $$2MeSH$$aLuminescence
000062894 650_2 $$2MeSH$$aModels, Biological
000062894 650_2 $$2MeSH$$aP-Glycoprotein: chemistry
000062894 650_2 $$2MeSH$$aP-Glycoproteins: chemistry
000062894 650_2 $$2MeSH$$aPlant Roots: metabolism
000062894 650_2 $$2MeSH$$aProtein Binding
000062894 650_2 $$2MeSH$$aTacrolimus Binding Proteins: chemistry
000062894 650_7 $$00$$2NLM Chemicals$$aATP-Binding Cassette Transporters
000062894 650_7 $$00$$2NLM Chemicals$$aATPGP1 protein, Arabidopsis
000062894 650_7 $$00$$2NLM Chemicals$$aArabidopsis Proteins
000062894 650_7 $$00$$2NLM Chemicals$$aFlavonoids
000062894 650_7 $$00$$2NLM Chemicals$$aIndoleacetic Acids
000062894 650_7 $$00$$2NLM Chemicals$$aP-Glycoprotein
000062894 650_7 $$00$$2NLM Chemicals$$aP-Glycoproteins
000062894 650_7 $$00$$2NLM Chemicals$$aTWD1 protein, Arabidopsis
000062894 650_7 $$0EC 5.2.1.-$$2NLM Chemicals$$aTacrolimus Binding Proteins
000062894 650_7 $$0EC 5.2.1.8$$2NLM Chemicals$$aImmunophilins
000062894 650_7 $$2WoSType$$aJ
000062894 7001_ $$0P:(DE-HGF)0$$aSovero, V.$$b1
000062894 7001_ $$0P:(DE-HGF)0$$aVincenzetti, V.$$b2
000062894 7001_ $$0P:(DE-HGF)0$$aSantelia, D.$$b3
000062894 7001_ $$0P:(DE-Juel1)VDB65461$$aBartnik, D.$$b4$$uFZJ
000062894 7001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b5$$uFZJ
000062894 7001_ $$0P:(DE-HGF)0$$aMancuso, S.$$b6
000062894 7001_ $$0P:(DE-HGF)0$$aMartinoia, E.$$b7
000062894 7001_ $$0P:(DE-HGF)0$$aGeisler, M.$$b8
000062894 773__ $$0PERI:(DE-600)1474604-9$$a10.1074/jbc.M709655200$$gVol. 283, p. 21817 - 21826$$p21817 - 21826$$q283<21817 - 21826$$tThe @journal of biological chemistry$$v283$$x0021-9258$$y2008
000062894 8567_ $$uhttp://dx.doi.org/10.1074/jbc.M709655200
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000062894 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000062894 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
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