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000062912 017__ $$aCopyright © American Society of Plant Biologists . This version is available at http://dx.doi.org/10.1104/pp.107.109215
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000062912 0247_ $$2DOI$$a10.1104/pp.107.109215
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000062912 084__ $$2WoS$$aPlant Sciences
000062912 1001_ $$0P:(DE-Juel1)129360$$aMetzner, R.$$b0$$uFZJ
000062912 245__ $$aImaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy
000062912 260__ $$aRockville, Md.: Soc.$$bJSTOR$$c2008
000062912 300__ $$a1774 - 1787
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000062912 440_0 $$04987$$aPlant Physiology$$v147$$x0032-0889$$y4
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000062912 520__ $$aA new approach to trace the transport routes of macronutrients in plants at the level of cells and tissues and to measure their elemental distributions was developed for investigating the dynamics and structure-function relationships of transport processes. Stem samples from Phaseolus vulgaris were used as a test system. Shock freezing and cryo-preparation were combined in a cryogenic chain with cryo-time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS) for element and isotope-specific imaging. Cryo-scanning electron microscopy (cryo-SEM) was integrated into the cryogenic workflow to assess the quality of structural preservation. We evaluated the capability of these techniques to monitor transport pathways and processes in xylem and associated tissues using supplementary sodium (Na) and tracers for potassium (K), rubidium (Rb), and (41)K added to the transpiration stream. Cryo-ToF-SIMS imaging produced detailed mappings of water, K, calcium, magnesium, the K tracers, and Na without quantification. Lateral resolutions ranged from 10 microm in survey mappings and at high mass resolution to approximately 1 microm in high lateral resolution imaging in reduced areas and at lower mass resolution. The tracers Rb and (41)K, as well as Na, were imaged with high sensitivity in xylem vessels and surrounding tissues. The isotope signature of the stable isotope tracer was utilized for relative quantification of the (41)K tracer as a fraction of total K at the single pixel level. Cryo-SEM confirmed that tissue structures had been preserved with subcellular detail throughout all procedures. Overlays of cryo-ToF-SIMS images onto the corresponding SEM images allowed detailed correlation of nutrient images with subcellular structures.
000062912 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000062912 588__ $$aDataset connected to Web of Science, Pubmed
000062912 650_2 $$2MeSH$$aBiological Transport
000062912 650_2 $$2MeSH$$aCryoelectron Microscopy: methods
000062912 650_2 $$2MeSH$$aMicroscopy, Electron, Scanning: methods
000062912 650_2 $$2MeSH$$aPhaseolus: chemistry
000062912 650_2 $$2MeSH$$aPhaseolus: metabolism
000062912 650_2 $$2MeSH$$aPhaseolus: ultrastructure
000062912 650_2 $$2MeSH$$aPlant Stems: chemistry
000062912 650_2 $$2MeSH$$aPlant Transpiration
000062912 650_2 $$2MeSH$$aPotassium: analysis
000062912 650_2 $$2MeSH$$aPotassium: metabolism
000062912 650_2 $$2MeSH$$aPotassium Isotopes
000062912 650_2 $$2MeSH$$aRubidium: analysis
000062912 650_2 $$2MeSH$$aRubidium: metabolism
000062912 650_2 $$2MeSH$$aSodium: analysis
000062912 650_2 $$2MeSH$$aSodium: metabolism
000062912 650_2 $$2MeSH$$aSpectrometry, Mass, Secondary Ion: methods
000062912 650_7 $$00$$2NLM Chemicals$$aPotassium Isotopes
000062912 650_7 $$07440-09-7$$2NLM Chemicals$$aPotassium
000062912 650_7 $$07440-17-7$$2NLM Chemicals$$aRubidium
000062912 650_7 $$07440-23-5$$2NLM Chemicals$$aSodium
000062912 650_7 $$2WoSType$$aJ
000062912 7001_ $$0P:(DE-Juel1)129397$$aSchneider, H. U.$$b1$$uFZJ
000062912 7001_ $$0P:(DE-Juel1)VDB2782$$aBreuer, U.$$b2$$uFZJ
000062912 7001_ $$0P:(DE-Juel1)VDB1472$$aSchröder, W. H.$$b3$$uFZJ
000062912 773__ $$0PERI:(DE-600)2004346-6$$a10.1104/pp.107.109215$$gVol. 147, p. 1774 - 1787$$p1774 - 1787$$q147<1774 - 1787$$tPlant physiology$$v147$$x0032-0889$$y2008
000062912 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2492657
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