TY  - JOUR
AU  - Metzner, R.
AU  - Schneider, H. U.
AU  - Breuer, U.
AU  - Schröder, W. H.
TI  - Imaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy
JO  - Plant physiology
VL  - 147
SN  - 0032-0889
CY  - Rockville, Md.: Soc.
PB  - JSTOR
M1  - PreJuSER-62912
SP  - 1774 - 1787
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - A new approach to trace the transport routes of macronutrients in plants at the level of cells and tissues and to measure their elemental distributions was developed for investigating the dynamics and structure-function relationships of transport processes. Stem samples from Phaseolus vulgaris were used as a test system. Shock freezing and cryo-preparation were combined in a cryogenic chain with cryo-time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS) for element and isotope-specific imaging. Cryo-scanning electron microscopy (cryo-SEM) was integrated into the cryogenic workflow to assess the quality of structural preservation. We evaluated the capability of these techniques to monitor transport pathways and processes in xylem and associated tissues using supplementary sodium (Na) and tracers for potassium (K), rubidium (Rb), and (41)K added to the transpiration stream. Cryo-ToF-SIMS imaging produced detailed mappings of water, K, calcium, magnesium, the K tracers, and Na without quantification. Lateral resolutions ranged from 10 microm in survey mappings and at high mass resolution to approximately 1 microm in high lateral resolution imaging in reduced areas and at lower mass resolution. The tracers Rb and (41)K, as well as Na, were imaged with high sensitivity in xylem vessels and surrounding tissues. The isotope signature of the stable isotope tracer was utilized for relative quantification of the (41)K tracer as a fraction of total K at the single pixel level. Cryo-SEM confirmed that tissue structures had been preserved with subcellular detail throughout all procedures. Overlays of cryo-ToF-SIMS images onto the corresponding SEM images allowed detailed correlation of nutrient images with subcellular structures.
KW  - Biological Transport
KW  - Cryoelectron Microscopy: methods
KW  - Microscopy, Electron, Scanning: methods
KW  - Phaseolus: chemistry
KW  - Phaseolus: metabolism
KW  - Phaseolus: ultrastructure
KW  - Plant Stems: chemistry
KW  - Plant Transpiration
KW  - Potassium: analysis
KW  - Potassium: metabolism
KW  - Potassium Isotopes
KW  - Rubidium: analysis
KW  - Rubidium: metabolism
KW  - Sodium: analysis
KW  - Sodium: metabolism
KW  - Spectrometry, Mass, Secondary Ion: methods
KW  - Potassium Isotopes (NLM Chemicals)
KW  - Potassium (NLM Chemicals)
KW  - Rubidium (NLM Chemicals)
KW  - Sodium (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18567833
C2  - pmc:PMC2492657
UR  - <Go to ISI:>//WOS:000258184800029
DO  - DOI:10.1104/pp.107.109215
UR  - https://juser.fz-juelich.de/record/62912
ER  -