% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Metzner:62912,
      author       = {Metzner, R. and Schneider, H. U. and Breuer, U. and
                      Schröder, W. H.},
      title        = {{I}maging {N}utrient {D}istributions in {P}lant {T}issue
                      {U}sing {T}ime-of-{F}light {S}econdary {I}on {M}ass
                      {S}pectrometry and {S}canning {E}lectron {M}icroscopy},
      journal      = {Plant physiology},
      volume       = {147},
      issn         = {0032-0889},
      address      = {Rockville, Md.: Soc.},
      publisher    = {JSTOR},
      reportid     = {PreJuSER-62912},
      pages        = {1774 - 1787},
      year         = {2008},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A new approach to trace the transport routes of
                      macronutrients in plants at the level of cells and tissues
                      and to measure their elemental distributions was developed
                      for investigating the dynamics and structure-function
                      relationships of transport processes. Stem samples from
                      Phaseolus vulgaris were used as a test system. Shock
                      freezing and cryo-preparation were combined in a cryogenic
                      chain with cryo-time-of-flight secondary ion mass
                      spectrometry (cryo-ToF-SIMS) for element and
                      isotope-specific imaging. Cryo-scanning electron microscopy
                      (cryo-SEM) was integrated into the cryogenic workflow to
                      assess the quality of structural preservation. We evaluated
                      the capability of these techniques to monitor transport
                      pathways and processes in xylem and associated tissues using
                      supplementary sodium (Na) and tracers for potassium (K),
                      rubidium (Rb), and (41)K added to the transpiration stream.
                      Cryo-ToF-SIMS imaging produced detailed mappings of water,
                      K, calcium, magnesium, the K tracers, and Na without
                      quantification. Lateral resolutions ranged from 10 microm in
                      survey mappings and at high mass resolution to approximately
                      1 microm in high lateral resolution imaging in reduced areas
                      and at lower mass resolution. The tracers Rb and (41)K, as
                      well as Na, were imaged with high sensitivity in xylem
                      vessels and surrounding tissues. The isotope signature of
                      the stable isotope tracer was utilized for relative
                      quantification of the (41)K tracer as a fraction of total K
                      at the single pixel level. Cryo-SEM confirmed that tissue
                      structures had been preserved with subcellular detail
                      throughout all procedures. Overlays of cryo-ToF-SIMS images
                      onto the corresponding SEM images allowed detailed
                      correlation of nutrient images with subcellular structures.},
      keywords     = {Biological Transport / Cryoelectron Microscopy: methods /
                      Microscopy, Electron, Scanning: methods / Phaseolus:
                      chemistry / Phaseolus: metabolism / Phaseolus:
                      ultrastructure / Plant Stems: chemistry / Plant
                      Transpiration / Potassium: analysis / Potassium: metabolism
                      / Potassium Isotopes / Rubidium: analysis / Rubidium:
                      metabolism / Sodium: analysis / Sodium: metabolism /
                      Spectrometry, Mass, Secondary Ion: methods / Potassium
                      Isotopes (NLM Chemicals) / Potassium (NLM Chemicals) /
                      Rubidium (NLM Chemicals) / Sodium (NLM Chemicals) / J
                      (WoSType)},
      cin          = {ZCH / ICG-3},
      ddc          = {580},
      cid          = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)ICG-3-20090406},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:18567833},
      pmc          = {pmc:PMC2492657},
      UT           = {WOS:000258184800029},
      doi          = {10.1104/pp.107.109215},
      url          = {https://juser.fz-juelich.de/record/62912},
}