Home > Publications database > Imaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy > print

001     62912
005     20200423204622.0
017 _ _ |a Copyright © American Society of Plant Biologists . This version is available at http://dx.doi.org/10.1104/pp.107.109215
024 7 _ |a pmid:18567833
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024 7 _ |a pmc:PMC2492657
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024 7 _ |a 10.1104/pp.107.109215
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024 7 _ |a WOS:000258184800029
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024 7 _ |a 2128/3352
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037 _ _ |a PreJuSER-62912
041 _ _ |a eng
082 _ _ |a 580
084 _ _ |2 WoS
|a Plant Sciences
100 1 _ |a Metzner, R.
|b 0
|u FZJ
|0 P:(DE-Juel1)129360
245 _ _ |a Imaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy
260 _ _ |a Rockville, Md.: Soc.
|b JSTOR
|c 2008
300 _ _ |a 1774 - 1787
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a article
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440 _ 0 |a Plant Physiology
|x 0032-0889
|0 4987
|y 4
|v 147
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a A new approach to trace the transport routes of macronutrients in plants at the level of cells and tissues and to measure their elemental distributions was developed for investigating the dynamics and structure-function relationships of transport processes. Stem samples from Phaseolus vulgaris were used as a test system. Shock freezing and cryo-preparation were combined in a cryogenic chain with cryo-time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS) for element and isotope-specific imaging. Cryo-scanning electron microscopy (cryo-SEM) was integrated into the cryogenic workflow to assess the quality of structural preservation. We evaluated the capability of these techniques to monitor transport pathways and processes in xylem and associated tissues using supplementary sodium (Na) and tracers for potassium (K), rubidium (Rb), and (41)K added to the transpiration stream. Cryo-ToF-SIMS imaging produced detailed mappings of water, K, calcium, magnesium, the K tracers, and Na without quantification. Lateral resolutions ranged from 10 microm in survey mappings and at high mass resolution to approximately 1 microm in high lateral resolution imaging in reduced areas and at lower mass resolution. The tracers Rb and (41)K, as well as Na, were imaged with high sensitivity in xylem vessels and surrounding tissues. The isotope signature of the stable isotope tracer was utilized for relative quantification of the (41)K tracer as a fraction of total K at the single pixel level. Cryo-SEM confirmed that tissue structures had been preserved with subcellular detail throughout all procedures. Overlays of cryo-ToF-SIMS images onto the corresponding SEM images allowed detailed correlation of nutrient images with subcellular structures.
536 _ _ |a Terrestrische Umwelt
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Biological Transport
650 _ 2 |2 MeSH
|a Cryoelectron Microscopy: methods
650 _ 2 |2 MeSH
|a Microscopy, Electron, Scanning: methods
650 _ 2 |2 MeSH
|a Phaseolus: chemistry
650 _ 2 |2 MeSH
|a Phaseolus: metabolism
650 _ 2 |2 MeSH
|a Phaseolus: ultrastructure
650 _ 2 |2 MeSH
|a Plant Stems: chemistry
650 _ 2 |2 MeSH
|a Plant Transpiration
650 _ 2 |2 MeSH
|a Potassium: analysis
650 _ 2 |2 MeSH
|a Potassium: metabolism
650 _ 2 |2 MeSH
|a Potassium Isotopes
650 _ 2 |2 MeSH
|a Rubidium: analysis
650 _ 2 |2 MeSH
|a Rubidium: metabolism
650 _ 2 |2 MeSH
|a Sodium: analysis
650 _ 2 |2 MeSH
|a Sodium: metabolism
650 _ 2 |2 MeSH
|a Spectrometry, Mass, Secondary Ion: methods
650 _ 7 |0 0
|2 NLM Chemicals
|a Potassium Isotopes
650 _ 7 |0 7440-09-7
|2 NLM Chemicals
|a Potassium
650 _ 7 |0 7440-17-7
|2 NLM Chemicals
|a Rubidium
650 _ 7 |0 7440-23-5
|2 NLM Chemicals
|a Sodium
650 _ 7 |a J
|2 WoSType
700 1 _ |a Schneider, H. U.
|b 1
|u FZJ
|0 P:(DE-Juel1)129397
700 1 _ |a Breuer, U.
|b 2
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|0 P:(DE-Juel1)VDB2782
700 1 _ |a Schröder, W. H.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB1472
773 _ _ |a 10.1104/pp.107.109215
|g Vol. 147, p. 1774 - 1787
|p 1774 - 1787
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|t Plant physiology
|v 147
|y 2008
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856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2492657
856 4 _ |u https://juser.fz-juelich.de/record/62912/files/Metzner.pdf
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