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000062913 0247_ $$2pmid$$apmid:18192444
000062913 0247_ $$2pmc$$apmc:PMC2259038
000062913 0247_ $$2DOI$$a10.1104/pp.107.111237
000062913 0247_ $$2WOS$$aWOS:000256412200031
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000062913 041__ $$aeng
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000062913 084__ $$2WoS$$aPlant Sciences
000062913 1001_ $$0P:(DE-HGF)0$$aJonczyk, R.$$b0
000062913 245__ $$aElucidation of the Final Reactions of DIMBOA-Glucoside Biosysnthesis in Maize: Characterization of Bx6 and Bx71[W][OA]
000062913 260__ $$aRockville, Md.: Soc.$$bJSTOR$$c2008
000062913 300__ $$a1053 - 1063
000062913 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000062913 3367_ $$2DataCite$$aOutput Types/Journal article
000062913 3367_ $$00$$2EndNote$$aJournal Article
000062913 3367_ $$2BibTeX$$aARTICLE
000062913 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000062913 3367_ $$2DRIVER$$aarticle
000062913 440_0 $$04987$$aPlant Physiology$$v146$$x0032-0889$$y3
000062913 500__ $$aRecord converted from VDB: 12.11.2012
000062913 520__ $$aBenzoxazinoids were identified in the early 1960s as secondary metabolites of the grasses that function as natural pesticides and exhibit allelopathic properties. Benzoxazinoids are synthesized in seedlings and stored as glucosides (glcs); the main aglucone moieties are 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) and 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA). The genes of DIBOA-glc biosynthesis have previously been isolated and the enzymatic functions characterized. Here, the enzymes for conversion of DIBOA-glc to DIMBOA-glc are identified. DIBOA-glc is the substrate of the dioxygenase BENZOXAZINLESS6 (BX6) and the produced 2,4,7-trihydroxy-2H-1,4-benzoxazin-3-(4H)-one-glc is metabolized by the methyltransferase BX7 to yield DIMBOA-glc. Both enzymes exhibit moderate K(m) values (below 0.4 mm) and k(cat) values of 2.10 s(-1) and 0.25 s(-1), respectively. Although BX6 uses a glucosylated substrate, our localization studies indicate a cytoplasmic localization of the dioxygenase. Bx6 and Bx7 are highest expressed in seedling tissue, a feature shared with the other Bx genes. At present, Bx6 and Bx7 have no close relatives among the members of their respective gene families. Bx6 and Bx7 map to the cluster of Bx genes on the short arm of chromosome 4.
000062913 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000062913 588__ $$aDataset connected to Web of Science, Pubmed
000062913 650_2 $$2MeSH$$aBenzoxazines: metabolism
000062913 650_2 $$2MeSH$$aChromosomes, Plant
000062913 650_2 $$2MeSH$$aCytoplasm: enzymology
000062913 650_2 $$2MeSH$$aGenes, Plant
000062913 650_2 $$2MeSH$$aGlucosides: biosynthesis
000062913 650_2 $$2MeSH$$aGlucosides: metabolism
000062913 650_2 $$2MeSH$$aMolecular Sequence Data
000062913 650_2 $$2MeSH$$aMultigene Family
000062913 650_2 $$2MeSH$$aPhylogeny
000062913 650_2 $$2MeSH$$aProtein O-Methyltransferase: genetics
000062913 650_2 $$2MeSH$$aProtein O-Methyltransferase: metabolism
000062913 650_2 $$2MeSH$$aSeedling: enzymology
000062913 650_2 $$2MeSH$$aZea mays: enzymology
000062913 650_2 $$2MeSH$$aZea mays: genetics
000062913 650_7 $$00$$2NLM Chemicals$$a2,4-dihydroxy-1,4-benzoxazin-3-one
000062913 650_7 $$00$$2NLM Chemicals$$a2-O-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one
000062913 650_7 $$00$$2NLM Chemicals$$aBenzoxazines
000062913 650_7 $$00$$2NLM Chemicals$$aGlucosides
000062913 650_7 $$015893-52-4$$2NLM Chemicals$$a2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one
000062913 650_7 $$0EC 2.1.1.-$$2NLM Chemicals$$aProtein O-Methyltransferase
000062913 650_7 $$2WoSType$$aJ
000062913 7001_ $$0P:(DE-HGF)0$$aSchmidt, H.$$b1
000062913 7001_ $$0P:(DE-HGF)0$$aOsterrieder, A.$$b2
000062913 7001_ $$0P:(DE-HGF)0$$aFiesselmann, A.$$b3
000062913 7001_ $$0P:(DE-HGF)0$$aSchullehner, K.$$b4
000062913 7001_ $$0P:(DE-HGF)0$$aHaslbeck, M.$$b5
000062913 7001_ $$0P:(DE-HGF)0$$aSicker, D.$$b6
000062913 7001_ $$0P:(DE-Juel1)129471$$aHofmann, D.$$b7$$uFZJ
000062913 7001_ $$0P:(DE-HGF)0$$aYalpani, N.$$b8
000062913 7001_ $$0P:(DE-HGF)0$$aSimmons, C.$$b9
000062913 7001_ $$0P:(DE-HGF)0$$aFrey, M.$$b10
000062913 7001_ $$0P:(DE-HGF)0$$aGierl, A.$$b11
000062913 773__ $$0PERI:(DE-600)2004346-6$$a10.1104/pp.107.111237$$gVol. 146, p. 1053 - 1063$$p1053 - 1063$$q146<1053 - 1063$$tPlant physiology$$v146$$x0032-0889$$y2008
000062913 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259038
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000062913 9141_ $$y2008
000062913 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
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