TY  - JOUR
AU  - Wittlich, M.
AU  - Koenig, B. W.
AU  - Stoldt, M.
AU  - Schmidt, H.
AU  - Willbold, D.
TI  - NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles
JO  - The FEBS journal
VL  - 276
SN  - 1742-464X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-6550
SP  - 6560 - 6575
PY  - 2009
N1  - This work was supported by a grant from the Prasidentenfond der Helmholtzgemeinschaft (HGF, Virtual Institute of Structural Biology) to DW.
AB  - The HIV-1 encoded virus protein U (VpU) is required for efficient viral release from human host cells and for induction of CD4 degradation in the endoplasmic reticulum. The cytoplasmic domain of the membrane protein VpU (VpUcyt) is essential for the latter activity. The structure and dynamics of VpUcyt were characterized in the presence of membrane simulating dodecylphosphatidylcholine (DPC) micelles by high-resolution liquid state NMR. VpUcyt is unstructured in aqueous buffer. The addition of DPC micelles induces a well-defined membrane proximal alpha-helix (residues I39-E48) and an additional helical segment (residues L64-R70). A tight loop (L73-V78) is observed close to the C-terminus, whereas the interhelical linker (R49-E63) remains highly flexible. A 3D structure of VpUcyt in the presence of DPC micelles was calculated from a large set of proton-proton distance constraints. The topology of micelle-associated VpUcyt was derived from paramagnetic relaxation enhancement of protein nuclear spins after the introduction of paramagnetic probes into the interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemical shift and paramagnetic relaxation enhancement data in conjunction with dynamic information from heteronuclear NOEs and structural insight from homonuclear NOE-based distance constraints indicated that micelle-associated VpUcyt retains a substantial degree of structural flexibility.
KW  - HIV-1: chemistry
KW  - HIV-1: metabolism
KW  - Human Immunodeficiency Virus Proteins: chemistry
KW  - Magnetic Resonance Spectroscopy
KW  - Micelles
KW  - Phosphorylcholine: analogs & derivatives
KW  - Phosphorylcholine: chemistry
KW  - Protein Structure, Tertiary
KW  - Viral Regulatory and Accessory Proteins: chemistry
KW  - Human Immunodeficiency Virus Proteins (NLM Chemicals)
KW  - Micelles (NLM Chemicals)
KW  - Viral Regulatory and Accessory Proteins (NLM Chemicals)
KW  - vpu protein, Human immunodeficiency virus 1 (NLM Chemicals)
KW  - Phosphorylcholine (NLM Chemicals)
KW  - dodecylphosphocholine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19804408
UR  - <Go to ISI:>//WOS:000271057200015
DO  - DOI:10.1111/j.1742-4658.2009.07363.x
UR  - https://juser.fz-juelich.de/record/6550
ER  -