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000006761 0247_ $$2DOI$$a10.1104/pp.109.143776
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000006761 084__ $$2WoS$$aPlant Sciences
000006761 1001_ $$0P:(DE-Juel1)129360$$aMetzner, R.$$b0$$uFZJ
000006761 245__ $$aTracing cationic nutrients from xylem into stem tissue of Phaseolus vulgaris by stable isotope tracers and cryo-secondary ion mass spectrometry
000006761 260__ $$aRockville, Md.: Soc.$$bJSTOR$$c2010
000006761 300__ $$a1030 - 1043
000006761 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000006761 440_0 $$04987$$aPlant Physiology$$v152$$x0032-0889
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000006761 520__ $$aFluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration stream of cut bean (Phaseolus vulgaris) shoots to study their radial exchange at the cell and tissue level with stem tissues between pith and phloem. For isotope localization, we combined sample preparation with secondary ion mass spectrometry in a completely cryogenic workflow. After 20 min of application, tracers were readily detectable to various degrees in all tissues. The xylem parenchyma near the vessels exchanged freely with the vessels, its nutrient elements reaching a steady state of strong exchange with elements in the vessels within 20 min, mainly via apoplastic pathways. A slow exchange between vessels and cambium and phloem suggested that they are separated from the xylem, parenchyma, and pith, possibly by an apoplastic barrier to diffusion for nutrients (as for carbohydrates). There was little difference in these distributions when tracers were applied directly to intact xylem via a microcapillary, suggesting that xylem tension had little effect on radial exchange of these nutrients and that their movement was mainly diffusive.
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000006761 650_2 $$2MeSH$$aBiological Transport
000006761 650_2 $$2MeSH$$aCryoelectron Microscopy
000006761 650_2 $$2MeSH$$aIsotopes: chemistry
000006761 650_2 $$2MeSH$$aMicroscopy, Electron, Scanning
000006761 650_2 $$2MeSH$$aPhaseolus: chemistry
000006761 650_2 $$2MeSH$$aPlant Stems: ultrastructure
000006761 650_2 $$2MeSH$$aPlant Transpiration
000006761 650_2 $$2MeSH$$aSpectrometry, Mass, Secondary Ion: methods
000006761 650_2 $$2MeSH$$aXylem: chemistry
000006761 650_7 $$00$$2NLM Chemicals$$aIsotopes
000006761 650_7 $$2WoSType$$aJ
000006761 7001_ $$0P:(DE-Juel1)129397$$aSchneider, H. U.$$b1$$uFZJ
000006761 7001_ $$0P:(DE-Juel1)VDB2782$$aBreuer, U.$$b2$$uFZJ
000006761 7001_ $$0P:(DE-Juel1)VDB67249$$aThorpe, M.R.$$b3$$uFZJ
000006761 7001_ $$0P:(DE-Juel1)129402$$aSchurr, U.$$b4$$uFZJ
000006761 7001_ $$0P:(DE-Juel1)VDB1472$$aSchröder, W. H.$$b5$$uFZJ
000006761 773__ $$0PERI:(DE-600)2004346-6$$a10.1104/pp.109.143776$$gVol. 152, p. 1030 - 1043$$p1030 - 1043$$q152<1030 - 1043$$tPlant physiology$$v152$$x0032-0889$$y2010
000006761 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815875
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000006761 9132_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000006761 9141_ $$y2010
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