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@ARTICLE{Metzner:6761,
      author       = {Metzner, R. and Schneider, H. U. and Breuer, U. and Thorpe,
                      M.R. and Schurr, U. and Schröder, W. H.},
      title        = {{T}racing cationic nutrients from xylem into stem tissue of
                      {P}haseolus vulgaris by stable isotope tracers and
                      cryo-secondary ion mass spectrometry},
      journal      = {Plant physiology},
      volume       = {152},
      issn         = {0032-0889},
      address      = {Rockville, Md.: Soc.},
      publisher    = {JSTOR},
      reportid     = {PreJuSER-6761},
      pages        = {1030 - 1043},
      year         = {2010},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Fluxes of mineral nutrients in the xylem are strongly
                      influenced by interactions with the surrounding stem tissues
                      and are probably regulated by them. Toward a mechanistic
                      understanding of these interactions, we applied stable
                      isotope tracers of magnesium, potassium, and calcium
                      continuously to the transpiration stream of cut bean
                      (Phaseolus vulgaris) shoots to study their radial exchange
                      at the cell and tissue level with stem tissues between pith
                      and phloem. For isotope localization, we combined sample
                      preparation with secondary ion mass spectrometry in a
                      completely cryogenic workflow. After 20 min of application,
                      tracers were readily detectable to various degrees in all
                      tissues. The xylem parenchyma near the vessels exchanged
                      freely with the vessels, its nutrient elements reaching a
                      steady state of strong exchange with elements in the vessels
                      within 20 min, mainly via apoplastic pathways. A slow
                      exchange between vessels and cambium and phloem suggested
                      that they are separated from the xylem, parenchyma, and
                      pith, possibly by an apoplastic barrier to diffusion for
                      nutrients (as for carbohydrates). There was little
                      difference in these distributions when tracers were applied
                      directly to intact xylem via a microcapillary, suggesting
                      that xylem tension had little effect on radial exchange of
                      these nutrients and that their movement was mainly
                      diffusive.},
      keywords     = {Biological Transport / Cryoelectron Microscopy / Isotopes:
                      chemistry / Microscopy, Electron, Scanning / Phaseolus:
                      chemistry / Plant Stems: ultrastructure / Plant
                      Transpiration / Spectrometry, Mass, Secondary Ion: methods /
                      Xylem: chemistry / Isotopes (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-3 / JARA-ENERGY},
      ddc          = {580},
      cid          = {I:(DE-Juel1)ICG-3-20090406 / $I:(DE-82)080011_20140620$},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19965970},
      pmc          = {pmc:PMC2815875},
      UT           = {WOS:000274246600052},
      doi          = {10.1104/pp.109.143776},
      url          = {https://juser.fz-juelich.de/record/6761},
}