%0 Journal Article
%A Glück, J.M.
%A Wittlich, M.
%A Feuerstein, S.E.
%A Hoffmann, S.
%A Willbold, D.
%A Koenig, B. W.
%T Integral membrane proteins in nanodiscs can be studied by solution NMR spectroscopy
%J Journal of the American Chemical Society
%V 131
%@ 0002-7863
%C Washington, DC
%I American Chemical Society
%M PreJuSER-7145
%P 12060 - 12061
%D 2009
%Z We thank Matthias Stoldt and Rudolf Hartmann for expert assistance and discussions. This work was supported by a grant from the Helmholtz Society ("Virtual Institute for Structural Biology") to D.W.
%X We present a two-dimensional solution NMR spectrum of an integral membrane protein (IMP) in a nanodisc. Solution NMR relies on rapid isotropic tumbling of the analyte with correlation times in the nanosecond range. IMPs in a cellular membrane do not satisfy this condition. Previous liquid-state NMR studies on IMPs were conducted in organic solvent or artificial membrane mimicking particles like detergent micelles. Nanodiscs are relatively small (150 kDa), detergent-free model membranes that are suitable for functional reconstitution of IMPs. Nanodiscs allow solubilization of integral membrane proteins in a nearly native lipid bilayer environment. The 70 residue polypeptide CD4mut was incorporated into nanodiscs. CD4mut features one transmembrane helix. The aliphatic (1)H-(13)C HSQC spectrum of nanodiscs with inserted, ((13)C, (15)N)-labeled CD4mut exhibits reasonably dispersed protein and lipid NMR signals. Our results demonstrate that IMPs in nanodiscs are amenable to liquid-state NMR methodology.
%K Antigens, CD4: chemistry
%K Antigens, CD4: genetics
%K Humans
%K Magnetic Resonance Spectroscopy
%K Membrane Proteins: chemistry
%K Membrane Proteins: genetics
%K Membranes, Artificial
%K Nanostructures: chemistry
%K Solutions
%K Antigens, CD4 (NLM Chemicals)
%K Membrane Proteins (NLM Chemicals)
%K Membranes, Artificial (NLM Chemicals)
%K Solutions (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:19663495
%U <Go to ISI:>//WOS:000269379600020
%R 10.1021/ja904897p
%U https://juser.fz-juelich.de/record/7145