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000007145 0247_ $$2pmid$$apmid:19663495
000007145 0247_ $$2DOI$$a10.1021/ja904897p
000007145 0247_ $$2WOS$$aWOS:000269379600020
000007145 037__ $$aPreJuSER-7145
000007145 041__ $$aeng
000007145 082__ $$a540
000007145 084__ $$2WoS$$aChemistry, Multidisciplinary
000007145 1001_ $$0P:(DE-Juel1)VDB89237$$aGlück, J.M.$$b0$$uFZJ
000007145 245__ $$aIntegral membrane proteins in nanodiscs can be studied by solution NMR spectroscopy
000007145 260__ $$aWashington, DC$$bAmerican Chemical Society$$c2009
000007145 300__ $$a12060 - 12061
000007145 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000007145 3367_ $$2BibTeX$$aARTICLE
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000007145 440_0 $$08502$$aJournal of the American Chemical Society$$v131$$x0002-7863$$y34
000007145 500__ $$aWe thank Matthias Stoldt and Rudolf Hartmann for expert assistance and discussions. This work was supported by a grant from the Helmholtz Society ("Virtual Institute for Structural Biology") to D.W.
000007145 520__ $$aWe present a two-dimensional solution NMR spectrum of an integral membrane protein (IMP) in a nanodisc. Solution NMR relies on rapid isotropic tumbling of the analyte with correlation times in the nanosecond range. IMPs in a cellular membrane do not satisfy this condition. Previous liquid-state NMR studies on IMPs were conducted in organic solvent or artificial membrane mimicking particles like detergent micelles. Nanodiscs are relatively small (150 kDa), detergent-free model membranes that are suitable for functional reconstitution of IMPs. Nanodiscs allow solubilization of integral membrane proteins in a nearly native lipid bilayer environment. The 70 residue polypeptide CD4mut was incorporated into nanodiscs. CD4mut features one transmembrane helix. The aliphatic (1)H-(13)C HSQC spectrum of nanodiscs with inserted, ((13)C, (15)N)-labeled CD4mut exhibits reasonably dispersed protein and lipid NMR signals. Our results demonstrate that IMPs in nanodiscs are amenable to liquid-state NMR methodology.
000007145 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
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000007145 650_2 $$2MeSH$$aAntigens, CD4: chemistry
000007145 650_2 $$2MeSH$$aAntigens, CD4: genetics
000007145 650_2 $$2MeSH$$aHumans
000007145 650_2 $$2MeSH$$aMagnetic Resonance Spectroscopy
000007145 650_2 $$2MeSH$$aMembrane Proteins: chemistry
000007145 650_2 $$2MeSH$$aMembrane Proteins: genetics
000007145 650_2 $$2MeSH$$aMembranes, Artificial
000007145 650_2 $$2MeSH$$aNanostructures: chemistry
000007145 650_2 $$2MeSH$$aSolutions
000007145 650_7 $$00$$2NLM Chemicals$$aAntigens, CD4
000007145 650_7 $$00$$2NLM Chemicals$$aMembrane Proteins
000007145 650_7 $$00$$2NLM Chemicals$$aMembranes, Artificial
000007145 650_7 $$00$$2NLM Chemicals$$aSolutions
000007145 650_7 $$2WoSType$$aJ
000007145 7001_ $$0P:(DE-Juel1)VDB28257$$aWittlich, M.$$b1$$uFZJ
000007145 7001_ $$0P:(DE-Juel1)VDB89238$$aFeuerstein, S.E.$$b2$$uFZJ
000007145 7001_ $$0P:(DE-Juel1)VDB630$$aHoffmann, S.$$b3$$uFZJ
000007145 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b4$$uFZJ
000007145 7001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b5$$uFZJ
000007145 773__ $$0PERI:(DE-600)1472210-0$$a10.1021/ja904897p$$gVol. 131, p. 12060 - 12061$$p12060 - 12061$$q131<12060 - 12061$$tJournal of the American Chemical Society$$v131$$x0002-7863$$y2009
000007145 8567_ $$uhttp://dx.doi.org/10.1021/ja904897p
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000007145 9201_ $$0I:(DE-Juel1)VDB942$$d31.12.2010$$gISB$$kISB-3$$lStrukturbiochemie$$x0
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