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| 001 | 7145 | ||
| 005 | 20200402205724.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:19663495 |
| 024 | 7 | _ | |2 DOI |a 10.1021/ja904897p |
| 024 | 7 | _ | |2 WOS |a WOS:000269379600020 |
| 037 | _ | _ | |a PreJuSER-7145 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Chemistry, Multidisciplinary |
| 100 | 1 | _ | |a Glück, J.M. |b 0 |u FZJ |0 P:(DE-Juel1)VDB89237 |
| 245 | _ | _ | |a Integral membrane proteins in nanodiscs can be studied by solution NMR spectroscopy |
| 260 | _ | _ | |a Washington, DC |b American Chemical Society |c 2009 |
| 300 | _ | _ | |a 12060 - 12061 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of the American Chemical Society |x 0002-7863 |0 8502 |y 34 |v 131 |
| 500 | _ | _ | |a We thank Matthias Stoldt and Rudolf Hartmann for expert assistance and discussions. This work was supported by a grant from the Helmholtz Society ("Virtual Institute for Structural Biology") to D.W. |
| 520 | _ | _ | |a We present a two-dimensional solution NMR spectrum of an integral membrane protein (IMP) in a nanodisc. Solution NMR relies on rapid isotropic tumbling of the analyte with correlation times in the nanosecond range. IMPs in a cellular membrane do not satisfy this condition. Previous liquid-state NMR studies on IMPs were conducted in organic solvent or artificial membrane mimicking particles like detergent micelles. Nanodiscs are relatively small (150 kDa), detergent-free model membranes that are suitable for functional reconstitution of IMPs. Nanodiscs allow solubilization of integral membrane proteins in a nearly native lipid bilayer environment. The 70 residue polypeptide CD4mut was incorporated into nanodiscs. CD4mut features one transmembrane helix. The aliphatic (1)H-(13)C HSQC spectrum of nanodiscs with inserted, ((13)C, (15)N)-labeled CD4mut exhibits reasonably dispersed protein and lipid NMR signals. Our results demonstrate that IMPs in nanodiscs are amenable to liquid-state NMR methodology. |
| 536 | _ | _ | |a Funktion und Dysfunktion des Nervensystems |c P33 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK409 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Antigens, CD4: chemistry |
| 650 | _ | 2 | |2 MeSH |a Antigens, CD4: genetics |
| 650 | _ | 2 | |2 MeSH |a Humans |
| 650 | _ | 2 | |2 MeSH |a Magnetic Resonance Spectroscopy |
| 650 | _ | 2 | |2 MeSH |a Membrane Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a Membrane Proteins: genetics |
| 650 | _ | 2 | |2 MeSH |a Membranes, Artificial |
| 650 | _ | 2 | |2 MeSH |a Nanostructures: chemistry |
| 650 | _ | 2 | |2 MeSH |a Solutions |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Antigens, CD4 |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Membrane Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Membranes, Artificial |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Solutions |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Wittlich, M. |b 1 |u FZJ |0 P:(DE-Juel1)VDB28257 |
| 700 | 1 | _ | |a Feuerstein, S.E. |b 2 |u FZJ |0 P:(DE-Juel1)VDB89238 |
| 700 | 1 | _ | |a Hoffmann, S. |b 3 |u FZJ |0 P:(DE-Juel1)VDB630 |
| 700 | 1 | _ | |a Willbold, D. |b 4 |u FZJ |0 P:(DE-Juel1)132029 |
| 700 | 1 | _ | |a Koenig, B. W. |b 5 |u FZJ |0 P:(DE-Juel1)132009 |
| 773 | _ | _ | |a 10.1021/ja904897p |g Vol. 131, p. 12060 - 12061 |p 12060 - 12061 |q 131<12060 - 12061 |0 PERI:(DE-600)1472210-0 |t Journal of the American Chemical Society |v 131 |y 2009 |x 0002-7863 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1021/ja904897p |
| 909 | C | O | |o oai:juser.fz-juelich.de:7145 |p VDB |
| 913 | 1 | _ | |k P33 |v Funktion und Dysfunktion des Nervensystems |l Funktion und Dysfunktion des Nervensystems |b Gesundheit |0 G:(DE-Juel1)FUEK409 |x 0 |
| 914 | 1 | _ | |y 2009 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |d 31.12.2010 |g ISB |k ISB-3 |l Strukturbiochemie |0 I:(DE-Juel1)VDB942 |x 0 |
| 920 | 1 | _ | |0 I:(DE-82)080012_20140620 |k JARA-HPC |l Jülich Aachen Research Alliance - High-Performance Computing |g JARA |x 1 |
| 970 | _ | _ | |a VDB:(DE-Juel1)115674 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 980 | _ | _ | |a I:(DE-82)080012_20140620 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)VDB1346 |
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