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@ARTICLE{Feuerstein:7146,
author = {Feuerstein, S.E. and Pulvermüller, A. and Hartmann, R. and
Granzin, J. and Stoldt, M. and Henklein, P. and Ernst, O.P.
and Heck, M. and Willbold, D. and Koenig, B. W.},
title = {{H}elix {F}ormation in {A}rrestin {A}ccompanies
{R}ecognition of {P}hotoactivated {R}hodopsin},
journal = {Biochemistry},
volume = {48},
issn = {0006-2960},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {PreJuSER-7146},
pages = {10733 -10742},
year = {2009},
note = {This work was supported by grants from the Deutsche
Forschungsgemeinschaft (DFG) to B.W.K. (Ko 2143/3), AR (Pu
186/3), and O.P. E. (Er 294/1).},
abstract = {Binding of arrestin to photoactivated phosphorylated
rhodopsin terminates the amplification of visual signals in
photoreceptor cells. Currently, there is no crystal
structure of a rhodopsin-arrestin complex available,
although structures of unbound rhodopsin and arrestin have
been determined. High-affinity receptor binding is dependent
on distinct arrestin sites responsible for recognition of
rhodopsin activation and phosphorylation. The loop
connecting beta-strands V and VI in rod arrestin has been
implicated in the recognition of active rhodopsin. We report
the structure of receptor-bound arrestin peptide Arr(67-77)
mimicking this loop based on solution NMR data. The peptide
binds photoactivated rhodopsin in the unphosphorylated and
phosphorylated form with similar affinities and stabilizes
the metarhodopsin II photointermediate. A largely
alpha-helical conformation of the receptor-bound peptide is
observed.},
keywords = {Arrestin: chemistry / Models, Molecular / Nuclear Magnetic
Resonance, Biomolecular / Photochemistry / Protein
Conformation / Rhodopsin: chemistry / Arrestin (NLM
Chemicals) / Rhodopsin (NLM Chemicals) / J (WoSType)},
cin = {ISB-3},
ddc = {570},
cid = {I:(DE-Juel1)VDB942},
pnm = {Funktion und Dysfunktion des Nervensystems},
pid = {G:(DE-Juel1)FUEK409},
shelfmark = {Biochemistry $\&$ Molecular Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19835414},
UT = {WOS:000271459100009},
doi = {10.1021/bi900544p},
url = {https://juser.fz-juelich.de/record/7146},
}