Hauptseite > Publikationsdatenbank > NMR structure of the transmembrane and cytoplasmic domains of human CD4 in micelles > print

001     7147
005     20200402205724.0
024 7 _ |2 pmid
|a pmid:19781520
024 7 _ |2 DOI
|a 10.1016/j.bbamem.2009.09.010
024 7 _ |2 WOS
|a WOS:000274859300009
037 _ _ |a PreJuSER-7147
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
084 _ _ |2 WoS
|a Biophysics
100 1 _ |a Wittlich, M.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB28257
245 _ _ |a NMR structure of the transmembrane and cytoplasmic domains of human CD4 in micelles
260 _ _ |a Amsterdam [u.a.]
|b Elsevier
|c 2010
300 _ _ |a 122 - 127
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Biochimica et Biophysica Acta
|x 0006-3002
|0 802
|y 2
|v 1798
500 _ _ |a This work has been supported by a grant from the "Prasidentenfond der Helmholtzgemeinschaft" (HGF, "Virtual Institute of Structural Biology") to D.W.
520 _ _ |a The human cluster determinant 4 (CD4) is a type I transmembrane glycoprotein involved in T-cell signalling. It is expressed primarily on the surface of T helper cells but also on subsets of memory and regulatory T lymphocytes (CD4(+) cells). It serves as a coreceptor in T-cell receptor recognition of MHC II antigen complexes. Besides its cellular functions, CD4 serves as the main receptor for human immunodeficiency virus type I (HIV-1). During T-cell infection, the CD4 extracellular domain is bound by HIV-1 gp120, the viral surface glycoprotein, which triggers a number of conformational changes ultimately resulting in virion entry of the cell. Subsequently, CD4 is downregulated in infected cells by multiple strategies that involve direct interactions of the HIV-1 proteins VpU and Nef with the cytoplasmic part of CD4. In the present work, we describe the NOE-based solution structure of the transmembrane and cytoplasmic domains of the cystein-free variant of CD4 (CD4mut) in dodecylphosphocholine (DPC) micelles. Furthermore, we have characterized micelle-inserted CD4mut by paramagentic relaxation enhancement (PRE) agents and (1)H-(15)N heteronuclear NOE data. CD4mut features a stable and well-defined transmembrane helix from M372 to V395 buried in the micellar core and a cytoplasmic helix ranging from A404 to L413. Experimental data suggest the amphipathic cytoplasmic helix to be in close contact with the micellar surface. The role of the amphipathic helix and its interaction with the micellar surface is discussed with respect to the biological function of the full-length CD4 protein.
536 _ _ |a Funktion und Dysfunktion des Nervensystems
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536 _ _ |a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Antigens, CD4: chemistry
650 _ 2 |2 MeSH
|a Antigens, CD4: immunology
650 _ 2 |2 MeSH
|a Antigens, CD4: metabolism
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Micelles
650 _ 2 |2 MeSH
|a Nuclear Magnetic Resonance, Biomolecular: methods
650 _ 2 |2 MeSH
|a Phosphorylcholine: analogs & derivatives
650 _ 2 |2 MeSH
|a Phosphorylcholine: chemistry
650 _ 2 |2 MeSH
|a Phosphorylcholine: metabolism
650 _ 2 |2 MeSH
|a Protein Structure, Secondary: physiology
650 _ 2 |2 MeSH
|a Protein Structure, Tertiary: physiology
650 _ 7 |0 0
|2 NLM Chemicals
|a Antigens, CD4
650 _ 7 |0 0
|2 NLM Chemicals
|a Micelles
650 _ 7 |0 107-73-3
|2 NLM Chemicals
|a Phosphorylcholine
650 _ 7 |0 53949-18-1
|2 NLM Chemicals
|a dodecylphosphocholine
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a CD4
653 2 0 |2 Author
|a HIV-1
653 2 0 |2 Author
|a VpU
653 2 0 |2 Author
|a Membrane protein
653 2 0 |2 Author
|a NMR
700 1 _ |a Thiagarajan, P.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB89240
700 1 _ |a Koenig, B. W.
|b 2
|u FZJ
|0 P:(DE-Juel1)132009
700 1 _ |a Hartmann, R.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB57647
700 1 _ |a Willbold, D.
|b 4
|u FZJ
|0 P:(DE-Juel1)132029
773 _ _ |a 10.1016/j.bbamem.2009.09.010
|g Vol. 1798, p. 122 - 127
|p 122 - 127
|q 1798<122 - 127
|0 PERI:(DE-600)1460387-1
|t Biochimica et biophysica acta
|v 1798
|y 2010
|x 0006-3002
856 7 _ |u http://dx.doi.org/10.1016/j.bbamem.2009.09.010
909 C O |o oai:juser.fz-juelich.de:7147
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|v Physical Basis of Diseases
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914 1 _ |y 2010
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |d 31.12.2010
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