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000007510 0247_ $$2DOI$$a10.1002/prot.22477
000007510 0247_ $$2WOS$$aWOS:000270849900013
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000007510 041__ $$aeng
000007510 082__ $$a540
000007510 084__ $$2WoS$$aBiochemistry & Molecular Biology
000007510 084__ $$2WoS$$aBiophysics
000007510 1001_ $$0P:(DE-Juel1)VDB77156$$aThielmann, Y.$$b0$$uFZJ
000007510 245__ $$aComparative modeling of human NSF reveals a possible binding mode of GABARAP and GATE-16
000007510 260__ $$aNew York, NY$$bWiley-Liss$$c2009
000007510 300__ $$a637 - 646
000007510 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000007510 3367_ $$2BibTeX$$aARTICLE
000007510 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000007510 3367_ $$2DRIVER$$aarticle
000007510 440_0 $$021902$$aProteins: Structure, function and Bioinformatics$$v77$$x0887-3585$$y3
000007510 500__ $$aGrant sponsor: Deutsche Forschungsgememschaft (DFG); Grant number: Wil472/5.
000007510 520__ $$aVesicular trafficking is an important homeostatic process in eukaryotic cells which critically relies on membrane fusion. One of the essential components of the universal membrane fusion machinery is NSF (N-ethylmaleimide-sensitive factor), a large hexameric ATPase involved in disassembly of SNARE (soluble NSF attachment protein receptor) complexes. To improve our understanding of this sophisticated molecular machine, we have modeled the structure of the NSF hexamer in two alternative assemblies. Our data suggest a mechanistic concept of the operating mode of NSF which helps to explain the functional impact of post-translational modifications and mutations reported previously. Furthermore, we propose a binding site for the ubiquitin-like proteins GABARAP and GATE-16, which is supported by experimental evidence, yielding a complex with favorable surface complementarity.
000007510 536__ $$0G:(DE-Juel1)FUEK443$$2G:(DE-HGF)$$aProgramm Biosoft$$cN03$$x0
000007510 588__ $$aDataset connected to Web of Science, Pubmed
000007510 65320 $$2Author$$aN-ethylmaleimide-sensitive factor
000007510 65320 $$2Author$$aGABARAP
000007510 65320 $$2Author$$acomparative modeling
000007510 65320 $$2Author$$astructure model
000007510 65320 $$2Author$$aprotein-protein interaction
000007510 650_2 $$2MeSH$$aAdaptor Proteins, Signal Transducing: chemistry
000007510 650_2 $$2MeSH$$aAdenosine Triphosphate: chemistry
000007510 650_2 $$2MeSH$$aBinding Sites
000007510 650_2 $$2MeSH$$aHumans
000007510 650_2 $$2MeSH$$aHydrolysis
000007510 650_2 $$2MeSH$$aMicrofilament Proteins: chemistry
000007510 650_2 $$2MeSH$$aMicrotubule-Associated Proteins: chemistry
000007510 650_2 $$2MeSH$$aModels, Molecular
000007510 650_2 $$2MeSH$$aN-Ethylmaleimide-Sensitive Proteins: chemistry
000007510 650_2 $$2MeSH$$aPeptides: chemistry
000007510 650_2 $$2MeSH$$aPhosphorylation
000007510 650_2 $$2MeSH$$aProtein Binding
000007510 650_2 $$2MeSH$$aProtein Conformation
000007510 650_2 $$2MeSH$$aProtein Interaction Mapping
000007510 650_2 $$2MeSH$$aProtein Structure, Tertiary
000007510 650_2 $$2MeSH$$aUbiquitin: chemistry
000007510 650_7 $$00$$2NLM Chemicals$$aAdaptor Proteins, Signal Transducing
000007510 650_7 $$00$$2NLM Chemicals$$aGABARAP protein, human
000007510 650_7 $$00$$2NLM Chemicals$$aGABARAPL2 protein, human
000007510 650_7 $$00$$2NLM Chemicals$$aMicrofilament Proteins
000007510 650_7 $$00$$2NLM Chemicals$$aMicrotubule-Associated Proteins
000007510 650_7 $$00$$2NLM Chemicals$$aPeptides
000007510 650_7 $$00$$2NLM Chemicals$$aUbiquitin
000007510 650_7 $$056-65-5$$2NLM Chemicals$$aAdenosine Triphosphate
000007510 650_7 $$0EC 3.6.4.6$$2NLM Chemicals$$aN-Ethylmaleimide-Sensitive Proteins
000007510 650_7 $$2WoSType$$aJ
000007510 7001_ $$0P:(DE-Juel1)131988$$aWeiergräber, O.H.$$b1$$uFZJ
000007510 7001_ $$0P:(DE-Juel1)VDB84828$$aMa, P.$$b2$$uFZJ
000007510 7001_ $$0P:(DE-Juel1)132019$$aSchwarten, M.$$b3$$uFZJ
000007510 7001_ $$0P:(DE-Juel1)132012$$aMohrlüder, J.$$b4$$uFZJ
000007510 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b5$$uFZJ
000007510 773__ $$0PERI:(DE-600)1475032-6$$a10.1002/prot.22477$$gVol. 77, p. 637 - 646$$p637 - 646$$q77<637 - 646$$tProteins$$v77$$x0887-3585$$y2009
000007510 8567_ $$uhttp://dx.doi.org/10.1002/prot.22477
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000007510 9201_ $$0I:(DE-Juel1)ISB-2-20090406$$d31.12.2010$$gISB$$kISB-2$$lMolekulare Biophysik$$x0
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