TY  - JOUR
AU  - Thielmann, Y.
AU  - Weiergräber, O.H.
AU  - Ma, P.
AU  - Schwarten, M.
AU  - Mohrlüder, J.
AU  - Willbold, D.
TI  - Comparative modeling of human NSF reveals a possible binding mode of GABARAP and GATE-16
JO  - Proteins
VL  - 77
SN  - 0887-3585
CY  - New York, NY
PB  - Wiley-Liss
M1  - PreJuSER-7510
SP  - 637 - 646
PY  - 2009
N1  - Grant sponsor: Deutsche Forschungsgememschaft (DFG); Grant number: Wil472/5.
AB  - Vesicular trafficking is an important homeostatic process in eukaryotic cells which critically relies on membrane fusion. One of the essential components of the universal membrane fusion machinery is NSF (N-ethylmaleimide-sensitive factor), a large hexameric ATPase involved in disassembly of SNARE (soluble NSF attachment protein receptor) complexes. To improve our understanding of this sophisticated molecular machine, we have modeled the structure of the NSF hexamer in two alternative assemblies. Our data suggest a mechanistic concept of the operating mode of NSF which helps to explain the functional impact of post-translational modifications and mutations reported previously. Furthermore, we propose a binding site for the ubiquitin-like proteins GABARAP and GATE-16, which is supported by experimental evidence, yielding a complex with favorable surface complementarity.
KW  - Adaptor Proteins, Signal Transducing: chemistry
KW  - Adenosine Triphosphate: chemistry
KW  - Binding Sites
KW  - Humans
KW  - Hydrolysis
KW  - Microfilament Proteins: chemistry
KW  - Microtubule-Associated Proteins: chemistry
KW  - Models, Molecular
KW  - N-Ethylmaleimide-Sensitive Proteins: chemistry
KW  - Peptides: chemistry
KW  - Phosphorylation
KW  - Protein Binding
KW  - Protein Conformation
KW  - Protein Interaction Mapping
KW  - Protein Structure, Tertiary
KW  - Ubiquitin: chemistry
KW  - Adaptor Proteins, Signal Transducing (NLM Chemicals)
KW  - GABARAP protein, human (NLM Chemicals)
KW  - GABARAPL2 protein, human (NLM Chemicals)
KW  - Microfilament Proteins (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - Ubiquitin (NLM Chemicals)
KW  - Adenosine Triphosphate (NLM Chemicals)
KW  - N-Ethylmaleimide-Sensitive Proteins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19533740
UR  - <Go to ISI:>//WOS:000270849900013
DO  - DOI:10.1002/prot.22477
UR  - https://juser.fz-juelich.de/record/7510
ER  -