TY  - JOUR
AU  - Stadler, A.M.
AU  - Digel, I.
AU  - Artmann, G.M.
AU  - Embs, J.P.
AU  - Zaccai, G.
AU  - Büldt, G.
TI  - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
JO  - Biophysical journal
VL  - 95
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-763
SP  - 5449 - 5461
PY  - 2008
N1  - This research project was supported by the European Commission under the 6th Framework Programme through Key Action: Strengthening the European Research Area, Research Infrastructures, contract No. RII3-CT-2003505925.
AB  - A transition in hemoglobin behavior at close to body temperature has been discovered recently by micropipette aspiration experiments on single red blood cells (RBCs) and circular dichroism spectroscopy on hemoglobin solutions. The transition temperature was directly correlated to the body temperatures of a variety of species. In an exploration of the molecular basis for the transition, we present neutron scattering measurements of the temperature dependence of hemoglobin dynamics in whole human RBCs in vivo. The data reveal a change in the geometry of internal protein motions at 36.9 degrees C, at human body temperature. Above that temperature, amino acid side-chain motions occupy larger volumes than expected from normal temperature dependence, indicating partial unfolding of the protein. Global protein diffusion in RBCs was also measured and the findings compared favorably with theoretical predictions for short-time self-diffusion of noncharged hard-sphere colloids. The results demonstrated that changes in molecular dynamics in the picosecond time range and angstrom length scale might well be connected to a macroscopic effect on whole RBCs that occurs at body temperature.
KW  - Body Temperature
KW  - Diffusion
KW  - Elasticity
KW  - Erythrocytes: metabolism
KW  - Hemoglobins: metabolism
KW  - Humans
KW  - Neutron Diffraction
KW  - Protein Denaturation
KW  - Hemoglobins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18708462
C2  - pmc:PMC2586580
UR  - <Go to ISI:>//WOS:000260999500043
DO  - DOI:10.1529/biophysj.108.138040
UR  - https://juser.fz-juelich.de/record/763
ER  -