TY  - JOUR
AU  - Lanoue, A.
AU  - Burlat, V.
AU  - Henkes, G.J.
AU  - Koch, I.
AU  - Schurr, U.
AU  - Röse, U.S.R.
TI  - De novo biosynthesis of defense root exudates in response to Fusarium attack in barley
JO  - The new phytologist
VL  - 185
SN  - 0028-646X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-7810
SP  - 577 - 588
PY  - 2010
N1  - This work was supported by the EU-funded Marie-Curie training network BIORHIZ (Biotic interactions in the rhizosphere as structuring forces for plant communities, MRTN-CT-2003-505090). The authors gratefully acknowledge Dr Jurgen Wildt for his assistance in performing the <SUP>13</SUP>CO<INF>2</INF> experiment, Dr Gregoire Hummel for assistance and discussions, and Dr Arnd Kuhn for comments on an earlier version of the manuscript.
AB  - Despite recent advances in elucidation of natural products in root exudates, there are significant gaps in our understanding of the ecological significance of products in the rhizosphere. •  Here, we investigated the potential of barley (Hordeum vulgare) to secrete defense root exudates when challenged by the soilborne pathogen Fusarium graminearum. •  Liquid chromatography with photodiode array detection (LC-DAD) was used to profile induced small-molecular-weight exudates. Thus, t-cinnamic, p-coumaric, ferulic, syringic and vanillic acids were assigned to plant metabolism and were induced within 2 d after Fusarium inoculation. Biological tests demonstrated the ability of those induced root exudates to inhibit the germination of F. graminearum macroconidia. In vivo labeling experiments with 13CO2 revealed that the secreted t-cinnamic acid was synthesized de novo within 2 d of fungal infection. Simultaneously to its root exudation, t-cinnamic acid was accumulated in the roots. Microscopic analysis showed that nonlignin cell wall phenolics were induced not only in necrosed zones but in all root tissues. •  Results suggest that barley plants under attack respond by de novo biosynthesis and secretion of compounds with antimicrobial functions that may mediate natural disease resistance.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000272893800021
C6  - 20699651
DO  - DOI:10.1111/j.1469-8137.2009.03066.x
UR  - https://juser.fz-juelich.de/record/7810
ER  -