%0 Journal Article
%A Matusch, A.
%A Depboylu, C.
%A Palm, C.
%A Wu, B.
%A Höglinger, G.U.
%A Schäfer, M.K.-H.
%A Becker, J. S.
%T Cerebral Bioimaing of Cu, Fe, Zn, and Mn in the MPTP Mouse Model of Parkinson’s Disease Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS)
%J Journal of the American Society for Mass Spectrometry
%V 21
%@ 1044-0305
%C New York [u.a.]
%I Springer
%M PreJuSER-7995
%P 161 - 171
%D 2010
%Z The authors thank A. Zimmermann (Forschungszentrum Julich) for technical support with LA-ICP-MS measurements. This study was supported in part by the German Parkinson's Society and the University Hospitals of Giessen and Marburg, Germany.
%X Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson's disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated. We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to -10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain. The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson's disease.
%K 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine: pharmacology
%K Animals
%K Brain: drug effects
%K Brain: pathology
%K Brain Chemistry
%K Calibration
%K Copper: analysis
%K Equipment Design
%K Iron: analysis
%K Laser Therapy
%K Male
%K Manganese: analysis
%K Mass Spectrometry: instrumentation
%K Mass Spectrometry: methods
%K Metals, Heavy: analysis
%K Mice
%K Mice, Inbred C57BL
%K Neurotoxins: pharmacology
%K Parkinson Disease: metabolism
%K Zinc: analysis
%K Metals, Heavy (NLM Chemicals)
%K Neurotoxins (NLM Chemicals)
%K 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (NLM Chemicals)
%K Iron (NLM Chemicals)
%K Manganese (NLM Chemicals)
%K Copper (NLM Chemicals)
%K Zinc (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:19892565
%U <Go to ISI:>//WOS:000274518100021
%R 10.1016/j.jasms.2009.09.022
%U https://juser.fz-juelich.de/record/7995