TY  - JOUR
AU  - Matusch, A.
AU  - Depboylu, C.
AU  - Palm, C.
AU  - Wu, B.
AU  - Höglinger, G.U.
AU  - Schäfer, M.K.-H.
AU  - Becker, J. S.
TI  - Cerebral Bioimaing of Cu, Fe, Zn, and Mn in the MPTP Mouse Model of Parkinson’s Disease Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS)
JO  - Journal of the American Society for Mass Spectrometry
VL  - 21
SN  - 1044-0305
CY  - New York [u.a.]
PB  - Springer
M1  - PreJuSER-7995
SP  - 161 - 171
PY  - 2010
N1  - The authors thank A. Zimmermann (Forschungszentrum Julich) for technical support with LA-ICP-MS measurements. This study was supported in part by the German Parkinson's Society and the University Hospitals of Giessen and Marburg, Germany.
AB  - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson's disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated. We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to -10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain. The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson's disease.
KW  - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine: pharmacology
KW  - Animals
KW  - Brain: drug effects
KW  - Brain: pathology
KW  - Brain Chemistry
KW  - Calibration
KW  - Copper: analysis
KW  - Equipment Design
KW  - Iron: analysis
KW  - Laser Therapy
KW  - Male
KW  - Manganese: analysis
KW  - Mass Spectrometry: instrumentation
KW  - Mass Spectrometry: methods
KW  - Metals, Heavy: analysis
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Neurotoxins: pharmacology
KW  - Parkinson Disease: metabolism
KW  - Zinc: analysis
KW  - Metals, Heavy (NLM Chemicals)
KW  - Neurotoxins (NLM Chemicals)
KW  - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (NLM Chemicals)
KW  - Iron (NLM Chemicals)
KW  - Manganese (NLM Chemicals)
KW  - Copper (NLM Chemicals)
KW  - Zinc (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19892565
UR  - <Go to ISI:>//WOS:000274518100021
DO  - DOI:10.1016/j.jasms.2009.09.022
UR  - https://juser.fz-juelich.de/record/7995
ER  -