TY  - JOUR
AU  - Becker, J. S.
AU  - Zoriy, M.
AU  - Matusch, A.
AU  - Wu, B.
AU  - Salber, D.
AU  - Palm, C.
AU  - Becker, J. S.
TI  - Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
JO  - Mass spectrometry reviews
VL  - 29
SN  - 0277-7037
CY  - New York, NY [u.a.]
PB  - Wiley
M1  - PreJuSER-8020
SP  - 156 - 175
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - The distribution analysis of (essential, beneficial, or toxic) metals (e.g., Cu, Fe, Zn, Pb, and others), metalloids, and non-metals in biological tissues is of key interest in life science. Over the past few years, the development and application of several imaging mass spectrometric techniques has been rapidly growing in biology and medicine. Especially, in brain research metalloproteins are in the focus of targeted therapy approaches of neurodegenerative diseases such as Alzheimer's and Parkinson's disease, or stroke, or tumor growth. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) has been successfully applied as a powerful imaging (mapping) technique to produce quantitative images of detailed regionally specific element distributions in thin tissue sections of human or rodent brain. Imaging LA-ICP-QMS was also applied to investigate metal distributions in plant and animal sections to study, for example, the uptake and transport of nutrient and toxic elements or environmental contamination. The combination of imaging LA-ICP-MS of metals with proteomic studies using biomolecular mass spectrometry identifies metal-containing proteins and also phosphoproteins. Metal-containing proteins were imaged in a two-dimensional gel after electrophoretic separation of proteins (SDS or Blue Native PAGE). Recent progress in LA-ICP-MS imaging as a stand-alone technique and in combination with MALDI/ESI-MS for selected life science applications is summarized.
KW  - Animals
KW  - Brain Chemistry
KW  - Equipment Design
KW  - Humans
KW  - Laser Therapy: instrumentation
KW  - Laser Therapy: methods
KW  - Laser Therapy: trends
KW  - Mass Spectrometry: instrumentation
KW  - Mass Spectrometry: methods
KW  - Mass Spectrometry: trends
KW  - Metals: analysis
KW  - Neurodegenerative Diseases: pathology
KW  - Plants: chemistry
KW  - Metals (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19557838
UR  - <Go to ISI:>//WOS:000273518300007
DO  - DOI:10.1002/mas.20239
UR  - https://juser.fz-juelich.de/record/8020
ER  -