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@ARTICLE{Moudkov:807639,
      author       = {Moudříková, Šárka and Mojzeš, Peter and Zachleder,
                      Vilém and Pfaff, Christian and Behrendt, Dominik and
                      Nedbal, Ladislav},
      title        = {{R}aman and fluorescence microscopy sensing
                      energy-transducing and energy-storing structures in
                      microalgae},
      journal      = {Algal Research},
      volume       = {16},
      issn         = {2211-9264},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2016-02127},
      pages        = {224 - 232},
      year         = {2016},
      abstract     = {Chlorophylls and carotenoids are core components of
                      photosynthetic energy transduction in algal cells, whereas
                      starch, lipids, and polyphosphates represent energy
                      reserves. All these biomolecules exhibit characteristic
                      molecular vibrations that were sensed and localized in
                      individual cells of Desmodesmus quadricauda and Chlorella
                      vulgaris by confocal Raman microscopy. In the same cells,
                      fluorescence of chlorophylls and of lipid bodies stained
                      with Nile Red was mapped using the same instrument. In the
                      first of the three consecutive scans, a low-power and
                      short-exposure excitation by a green 532 nm laser was used
                      to map chlorophyll fluorescence. In the second scan, the
                      full power of a 785 nm laser was used for Raman mapping of
                      carotenoids. The third scan was performed again with the 532
                      nm laser to map non-fluorescent biomolecules such as lipids,
                      proteins, starch, and polyphosphates. Before the third scan,
                      chlorophyll fluorescence was suppressed by photobleaching a
                      wide area using a strongly defocused 532 nm laser beam.
                      Raman microscopy was used for the first time to localize
                      polyphosphate granules within a single algal cell in the
                      context of other relevant bio-energetic structures. The new
                      information represents an opportunity for algal cell
                      phenotyping.Abbreviations CCD, charge-coupled device; SVD,
                      singular value decompositionKeywords Biofuels; Lipids;
                      Photosynthesis; Pigments; Polyphosphate; Starch},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000375610000026},
      doi          = {10.1016/j.algal.2016.03.016},
      url          = {https://juser.fz-juelich.de/record/807639},
}