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@ARTICLE{Gapinski:808190,
author = {Gapinski, Jacek and Jarzębski, Maciej and Buitenhuis,
Johan and Deptula, Tobiasz and Mazuryk, Jaroslaw and
Patkowski, Adam},
title = {{S}tructure and dimensions of core-shell nanoparticles
comparable to the confocal volume studied by means of
fluorescence correlation spectroscopy},
journal = {Langmuir},
volume = {32},
number = {10},
issn = {1520-5827},
address = {Washington, DC},
publisher = {ACS Publ.},
reportid = {FZJ-2016-02216},
pages = {2482 - 2491},
year = {2016},
note = {I am only allowed to add the manuscript Version of the
publication after 19 February 2017, therfore, I will add the
full text manuscript Version after 19 February 2017.},
abstract = {In some applications the dye distribution within
fluorescently labeled nanoparticles and its stability over
long periods of time are important issues. In this article
we study numerically and experimentally the applicability of
fluorescence correlation spectroscopy (FCS) to resolve such
questions. When the size of fluorescently labeled particles
is comparable to or larger than the confocal volume, the
effective confocal volume seen in FCS experiments is
increasing. Such an effect has already been studied for
uniformly labeled spherical particles. In this work we
analyze the form of the FCS correlation functions (CFs) for
core-labeled and shell-labeled core–shell particles. For
shell-labeled particles an additional fast decay was found
both in simulations and in experiments on custom-made
surface-labeled particles. Universal scaling of FCS
correlation times based on the squared ratio of the labeled
part radius of gyration to the Gaussian radius of the beam
profile was found. Recipes based on the analysis of
simulated CFs, proposed for interpretation of experimental
results, were successfully applied to the FCS results on
suspensions of large core-labeled and surface-labeled
particles.},
cin = {ICS-3},
ddc = {670},
cid = {I:(DE-Juel1)ICS-3-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000372391600023},
doi = {10.1021/acs.langmuir.5b04605},
url = {https://juser.fz-juelich.de/record/808190},
}