TY  - JOUR
AU  - Zhang-Haagen, Bo
AU  - Biehl, Ralf
AU  - Nagel-Steger, Luitgard
AU  - Radulescu, Aurel
AU  - Richter, Dieter
AU  - Willbold, Dieter
TI  - Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering
JO  - PLoS one
VL  - 11
IS  - 2
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - FZJ-2016-02236
SP  - e0150267 -
PY  - 2016
AB  - Small proteins like amyloid beta (Aβ) monomers are related to neurodegenerative disorders by aggregation to insoluble fibrils. Small angle neutron scattering (SANS) is a nondestructive method to observe the aggregation process in solution. We show that SANS is able to resolve monomers of small molecular weight like Aβ for aggregation studies. We examine Aβ monomers after prolonged storing in d-hexafluoroisopropanol (dHFIP) by using SANS and dynamic light scattering (DLS). We determined the radius of gyration from SANS as 1.0±0.1 nm for Aβ1–40 and 1.6±0.1 nm for Aβ1–42 in agreement with 3D NMR structures in similar solvents suggesting a solvent surface layer with 5% increased density. After initial dissolution in dHFIP Aβ aggregates sediment with a major component of pure monomers showing a hydrodynamic radius of 1.8±0.3 nm for Aβ1–40 and 3.2±0.4 nm for Aβ1–42 including a surface layer of dHFIP solvent molecules.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000371274400114
C6  - pmid:26919121
DO  - DOI:10.1371/journal.pone.0150267
UR  - https://juser.fz-juelich.de/record/808413
ER  -