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@ARTICLE{Moreno:808415,
author = {Moreno, Angel J. and Lo Verso, Federica and Arbe, Arantxa
and Pomposo, José A. and Colmenero, Juan},
title = {{C}oncentrated {S}olutions of {S}ingle-{C}hain
{N}anoparticles: {A} {S}imple {M}odel for {I}ntrinsically
{D}isordered {P}roteins under {C}rowding {C}onditions},
journal = {The journal of physical chemistry letters},
volume = {7},
number = {5},
issn = {1948-7185},
address = {Washington, DC},
publisher = {ACS},
reportid = {FZJ-2016-02238},
pages = {838 - 844},
year = {2016},
abstract = {By means of large-scale computer simulations and
small-angle neutron scattering (SANS), we investigate
solutions of single-chain nanoparticles (SCNPs), covering
the whole concentration range from infinite dilution to melt
density. The analysis of the conformational properties of
the SCNPs reveals that these synthetic nano-objects share
basic ingredients with intrinsically disordered proteins
(IDPs), as topological polydispersity, generally sparse
conformations, and locally compact domains. We investigate
the role of the architecture of the SCNPs in their collapse
behavior under macromolecular crowding. Unlike in the case
of linear macromolecules, which experience the usual
transition from self-avoiding to Gaussian random-walk
conformations, crowding leads to collapsed conformations of
SCNPs resembling those of crumpled globules. This behavior
is already found at volume fractions (about $30\%)$ that are
characteristic of crowding in cellular environments. The
simulation results are confirmed by the SANS experiments.
Our results for SCNPs—a model system free of specific
interactions—propose a general scenario for the effect of
steric crowding on IDPs: collapse from sparse conformations
at high dilution to crumpled globular conformations in cell
environments.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II},
ddc = {530},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000371563700019},
doi = {10.1021/acs.jpclett.6b00144},
url = {https://juser.fz-juelich.de/record/808415},
}