guest ::
| Home > Publications database > Iodine-125-labeled DNA-Triplex-forming oligonucleotides reveal increased cyto- and genotoxic effectiveness compared to Phosphorus-32 > print |
| Home > Publications database > Iodine-125-labeled DNA-Triplex-forming oligonucleotides reveal increased cyto- and genotoxic effectiveness compared to Phosphorus-32 > print |
| 001 | 808536 | ||
| 005 | 20210129222806.0 | ||
| 024 | 7 | _ | |2 doi |a 10.3109/09553002.2016.1160157 |
| 024 | 7 | _ | |2 ISSN |a 0020-7616 |
| 024 | 7 | _ | |2 ISSN |a 0955-3002 |
| 024 | 7 | _ | |2 ISSN |a 1362-3095 |
| 024 | 7 | _ | |2 Handle |a 2128/16240 |
| 024 | 7 | _ | |2 WOS |a WOS:000388629800010 |
| 037 | _ | _ | |a FZJ-2016-02274 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |0 P:(DE-Juel1)133468 |a Dahmen, Volker |b 0 |u fzj |
| 245 | _ | _ | |a Iodine-125-labeled DNA-Triplex-forming oligonucleotides reveal increased cyto- and genotoxic effectiveness compared to Phosphorus-32 |
| 260 | _ | _ | |a London |b Taylor & Francis |c 2016 |
| 336 | 7 | _ | |2 DRIVER |a article |
| 336 | 7 | _ | |2 DataCite |a Output Types/Journal article |
| 336 | 7 | _ | |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |a Journal Article |b journal |m journal |s 1513263449_28639 |
| 336 | 7 | _ | |2 BibTeX |a ARTICLE |
| 336 | 7 | _ | |2 ORCID |a JOURNAL_ARTICLE |
| 336 | 7 | _ | |0 0 |2 EndNote |a Journal Article |
| 520 | _ | _ | |a PURPOSE: The efficacy of DNA-targeting radionuclide therapies might be strongly enhanced by employing short range particle-emitters. However, the gain of effectiveness is not yet well substantiated. We compared the Auger electron emitter I-125 to the ß--emitter P-32 in terms of biological effectiveness per decay and radiation dose when located in the close proximity to DNA using DNA Triplex-forming oligonucleotides (TFO). The clonogenicity and the induction of DNA double-strand breaks (DSB) were investigated in SCL-II cells after exposure to P-32- or I-125-labeled TFO targeting the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene and after external homogeneous exposure to gamma-rays as reference radiation.MATERIALS AND METHODS: TFO were labeled with P-32 or I-125 using the primer extension method. Cell survival was analyzed by colony-forming assay and DNA damage was assessed by microscopic quantification of protein 53 binding protein 1 (53BP1) foci in SCL-II cells.RESULTS: I-125-TFO induced a pronounced decrease of cell survival (D37 at ∼360 accumulated decays per cell, equivalent to 1.22 Gy cell nucleus dose) and a significant increase of 53BP1 foci with increasing decays. The P-32-labeled TFO induced neither a strong decrease of cell survival nor an increase of 53BP1 foci up to ∼4000 accumulated decays per cell, equivalent to ∼1 Gy cell nucleus dose. The RBE for I-125-TFO was in the range of 3-4 for both biological endpoints.CONCLUSIONS: I-125-TFO proved to be much more radiotoxic than P-32-TFO per decay and per unit dose although targeting the same sequence in the GAPDH gene. This might be well explained by the high number of low energy Auger electrons emitted by I-125 per decay, leading to a high ionization density in the immediate vicinity of the decay site, probably producing highly complex DNA lesions overcharging DNA repair mechanisms. |
| 536 | _ | _ | |0 G:(DE-HGF)POF3-899 |a 899 - ohne Topic (POF3-899) |c POF3-899 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 650 | 2 | 7 | |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |a Biology |x 0 |
| 700 | 1 | _ | |0 P:(DE-Juel1)133341 |a Pomplun, Ekkehard |b 1 |
| 700 | 1 | _ | |0 P:(DE-Juel1)133469 |a Kriehuber, Ralf |b 2 |e Corresponding author |
| 773 | _ | _ | |0 PERI:(DE-600)1498203-1 |a 10.3109/09553002.2016.1160157 |g p. 1 - 7 |n 11 |p 679-685 |t International journal of radiation biology |v 92 |x 0955-3002 |y 2016 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.gif?subformat=icon |x icon |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.jpg?subformat=icon-1440 |x icon-1440 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.jpg?subformat=icon-180 |x icon-180 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.jpg?subformat=icon-640 |x icon-640 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/808536/files/Abstract_Paper_Int-J_Radiat_Biol_2016.pdf?subformat=pdfa |x pdfa |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:808536 |p openaire |p open_access |p driver |p VDB |p dnbdelivery |
| 910 | 1 | _ | |0 I:(DE-588b)5008462-8 |6 P:(DE-Juel1)133468 |a Forschungszentrum Jülich |b 0 |k FZJ |
| 910 | 1 | _ | |0 I:(DE-588b)5008462-8 |6 P:(DE-Juel1)133341 |a Forschungszentrum Jülich |b 1 |k FZJ |
| 910 | 1 | _ | |0 I:(DE-588b)5008462-8 |6 P:(DE-Juel1)133469 |a Forschungszentrum Jülich GmbH |b 2 |k FZJ |
| 913 | 1 | _ | |0 G:(DE-HGF)POF3-899 |1 G:(DE-HGF)POF3-890 |2 G:(DE-HGF)POF3-800 |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |v ohne Topic |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2016 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0200 |2 StatID |a DBCoverage |b SCOPUS |
| 915 | _ | _ | |0 StatID:(DE-HGF)1030 |2 StatID |a DBCoverage |b Current Contents - Life Sciences |
| 915 | _ | _ | |0 StatID:(DE-HGF)0600 |2 StatID |a DBCoverage |b Ebsco Academic Search |
| 915 | _ | _ | |0 StatID:(DE-HGF)0100 |2 StatID |a JCR |b INT J RADIAT BIOL : 2015 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0150 |2 StatID |a DBCoverage |b Web of Science Core Collection |
| 915 | _ | _ | |0 StatID:(DE-HGF)0110 |2 StatID |a WoS |b Science Citation Index |
| 915 | _ | _ | |0 StatID:(DE-HGF)0111 |2 StatID |a WoS |b Science Citation Index Expanded |
| 915 | _ | _ | |0 StatID:(DE-HGF)9900 |2 StatID |a IF < 5 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0510 |2 StatID |a OpenAccess |
| 915 | _ | _ | |0 StatID:(DE-HGF)0030 |2 StatID |a Peer Review |b ASC |
| 915 | _ | _ | |0 StatID:(DE-HGF)0310 |2 StatID |a DBCoverage |b NCBI Molecular Biology Database |
| 915 | _ | _ | |0 StatID:(DE-HGF)1050 |2 StatID |a DBCoverage |b BIOSIS Previews |
| 915 | _ | _ | |0 StatID:(DE-HGF)0300 |2 StatID |a DBCoverage |b Medline |
| 915 | _ | _ | |0 StatID:(DE-HGF)0420 |2 StatID |a Nationallizenz |
| 915 | _ | _ | |0 StatID:(DE-HGF)0199 |2 StatID |a DBCoverage |b Thomson Reuters Master Journal List |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)S-US-20090406 |k S-US |l Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)S-US-20090406 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|