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@INPROCEEDINGS{Chlubek:810522,
author = {Chlubek, Antonia and Jahnke, Siegfried and Metzner, Ralf
and Pflugfelder, Daniel and Koller, Robert and Hombach,
Thomas},
title = {{L}abelling plants with radioactive 11{CO}2 for noninvasive
3{D} imaging with {PET}},
reportid = {FZJ-2016-03191},
year = {2016},
abstract = {Plant carbon dioxide fixation and subsequent
photoassimilates allocation are fundamentally important for
survival, growth and yield of plants. Since carbon
distribution in a plant is highly dynamic its investigation
is a unique challenge. Radiotracers, such as radioactive
carbon dioxide 11CO2 can be administered to a leaf or canopy
for tracing photoassimilate distribution within a plant. The
3D distribution of the 11C tracer can be monitored with a
positron emission tomograph (PET) in order to obtain carbon
transport parameters for functional phenotyping. For
labelling plants we established a gas exchange system for
both measuring gas exchange of leaves and administering
11CO2 to the plant. Handling the radioactive carbon dioxide
safely requires special precautions which are implemented in
the system. Here, we present results of the first gas
exchange measurements of pea (Pisum sativum) under drought
stress as well as images of 11C allocation into the root
measured with the PET system ‘PlanTIS’. For an improved
3D visualization of 11C transport a new PET system
(phenoPET) was constructed, which is currently under
evaluation. First experimental results on plants with both
phenoPET and the gas exchange system are expected by mid of
2016. In future, it is planned to automatize plant
transport, labeling and tracer measurement. All
installations combined will facilitate dynamic monitoring
and quantification of carbon assimilation with regards to
different phenotypes and under controlled environmental
conditions.},
month = {Mar},
date = {2016-03-14},
organization = {Plant 2030: Status Seminar 2016,
Potsdam (Germany), 14 Mar 2016 - 16 Mar
2016},
subtyp = {Invited},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)24},
url = {https://juser.fz-juelich.de/record/810522},
}