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@ARTICLE{Sharma:810932,
      author       = {Sharma, Kanika and Ahuja, Gaurav and Hussain, Ashiq and
                      Balfanz, Sabine and Baumann, A. and Korsching, Sigrun I.},
      title        = {{E}limination of a ligand gating site generates a
                      supersensitive olfactory receptor},
      journal      = {Scientific reports},
      volume       = {6},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2016-03498},
      pages        = {28359},
      year         = {2016},
      abstract     = {Olfaction poses one of the most complex ligand-receptor
                      matching problems in biology due to the unparalleled
                      multitude of odor molecules facing a large number of cognate
                      olfactory receptors. We have recently deorphanized an
                      olfactory receptor, TAAR13c, as a specific receptor for the
                      death-associated odor cadaverine. Here we have modeled the
                      cadaverine/TAAR13c interaction, exchanged predicted binding
                      residues by site-directed mutagenesis, and measured the
                      activity of the mutant receptors. Unexpectedly we observed a
                      binding site for cadaverine at the external surface of the
                      receptor, in addition to an internal binding site, whose
                      mutation resulted in complete loss of activity. In stark
                      contrast, elimination of the external binding site generated
                      supersensitive receptors. Modeling suggests this site to act
                      as a gate, limiting access of the ligand to the internal
                      binding site and thereby downregulating the affinity of the
                      native receptor. This constitutes a novel mechanism to
                      fine-tune physiological sensitivity to socially relevant
                      odors.},
      cin          = {ICS-4},
      ddc          = {000},
      cid          = {I:(DE-Juel1)ICS-4-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000378197600001},
      pubmed       = {pmid:27323929},
      doi          = {10.1038/srep28359},
      url          = {https://juser.fz-juelich.de/record/810932},
}