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@BOOK{Turtoi:811468,
      author       = {Turtoi, Andrei},
      title        = {{U}ntersuchung frühzeitiger {R}eaktionen lymphozytärer
                      {P}roteine auf {G}amma-{B}estrahlung humanen {V}ollbluts und
                      deren {D}osisabhängigkeit: {V}oraussetzung für die
                      {E}ntwicklung eines individuellen strahlenbiologischen
                      {D}osimeters},
      volume       = {40},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2016-03939},
      isbn         = {978-3-89336-502-9},
      series       = {Schriften des Forschungszentrums Jülich. Reihe
                      Lebenswissenschaften / Life Sciences},
      pages        = {97 S.},
      year         = {2007},
      abstract     = {The present thesis is concerned with the issues involved in
                      obtaining reliable experimental data permitting a
                      retrospective assessment of radiation-induced doses at the
                      time of application or contamination. In order to provide
                      prompt medical treatment of those injured in accidents with
                      ionizing radiation, biological procedures that can be
                      implemented swiftly and at an early stage are required both
                      to determine the radiation dose originally received as well
                      as to assess the course of the dosedependent biological
                      reactions on the basis of individual sensitivity to
                      radiation. To this end, in the present thesis the lymphocyte
                      proteins (phosphoproteins and total proteins) in blood taken
                      from test subjects who had been exposed to
                      $\gamma$-radiation (applied dose: 0-4 Gy) were analysed just
                      15 minutes after completing irradiation by means of 2D gel
                      electrophoresis. Only those early-response proteins (ERPROs)
                      that displayed a significant radiation-induced change were
                      identified by nano-HPLC-MS/MS. For validation purposes, the
                      dose-dependent gene expression of some of these proteins was
                      determined by RT-qPCR. The following ERPROs displayed
                      pronounced early reactions in the form of changes of
                      concentration in comparison to unirradiated control samples:
                      talin-1, talin-2, $\beta$-actin, mutant $\beta$-actin,
                      peroxin-1 and also the phosphoproteins annexin-A6,
                      MHC-binding protein-2, zyxin-2, interleukin-17E and
                      phosphoglycerate kinase-1. The majority of the lymphocyte
                      ERPROs represent proteins responsible for changes to the
                      cytoskeleton, proliferation and cell cycle, modulation of
                      immunoreactions as well as protein degradation and energy
                      production. Other cellular processes may not have been
                      determined due to the sensitivity restrictions of the
                      2D-PAGE and MS methods, but cannot be excluded. Gene
                      expression studies revealed that a combination of methods,
                      comprising RT-qPCR and 2D-PAGE as well as DNA microarray and
                      Western blot, may in future be able to overcome these
                      restrictions. The slopes of the curves from concentration
                      measurements of various early response proteins after doses
                      had been applied in the 0 and 4 Gy range yielded a
                      characteristic arrangement or pattern representative of an
                      individual. In case of contamination, this pattern prepared
                      ex vivo serves as a reference for identifying the originally
                      unknown dose, thus creating the necessary condition for
                      applying an individual radiation biodosimeter . This thus
                      provides for the first time an experimental means of
                      biologically quantifying in retrospect radioactive doses in
                      the 0 and 4 Gy range after a relatively short time.},
      cin          = {S-A},
      cid          = {I:(DE-Juel1)S-A-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)3},
      url          = {https://juser.fz-juelich.de/record/811468},
}