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@ARTICLE{Kallscheuer:811522,
      author       = {Kallscheuer, Nicolai and Vogt, Michael and Stenzel, Anton
                      and Gätgens, Jochem and Bott, Michael and Marienhagen, Jan},
      title        = {{C}onstruction of a {C}orynebacterium glutamicum platform
                      strain for the production of stilbenes and
                      (2{S})-flavanones},
      journal      = {Metabolic engineering},
      volume       = {38},
      issn         = {1096-7176},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2016-03979},
      pages        = {47 - 55},
      year         = {2016},
      note         = {Biotechnologie 1},
      abstract     = {Corynebacterium glutamicum is an important organism in
                      industrial biotechnology for the microbial production of
                      bulk chemicals, in particular amino acids. However, until
                      now activity of a complex catabolic network for the
                      degradation of aromatic compounds averted application of C.
                      glutamicum as production host for aromatic compounds of
                      pharmaceutical or biotechnological interest. In the course
                      of the construction of a suitable C. glutamicum platform
                      strain for plant polyphenol production, four gene clusters
                      comprising 21 genes involved in the catabolism of aromatic
                      compounds were deleted. Expression of plant-derived and
                      codon-optimized genes coding for a chalcone synthase (CHS)
                      and a chalcone isomerase (CHI) in this strain background
                      enabled formation of 35 mg/L naringenin and 37 mg/L
                      eriodictyol from the supplemented phenylpropanoids
                      p-coumaric acid and caffeic acid, respectively. Furthermore,
                      expression of genes coding for a 4-coumarate: CoA-ligase
                      (4CL) and a stilbene synthase (STS) led to the production of
                      the stilbenes pinosylvin, resveratrol and piceatannol
                      starting from supplemented phenylpropanoids cinnamic acid,
                      p-coumaric acid and caffeic acid, respectively. Stilbene
                      concentrations of up to 158 mg/L could be achieved.
                      Additional engineering of the amino acid metabolism for an
                      optimal connection to the synthetic plant polyphenol
                      pathways enabled resveratrol production directly from
                      glucose. The construction of these C. glutamicum platform
                      strains for the synthesis of plant polyphenols opens the
                      door towards the microbial production of high-value aromatic
                      compounds from cheap carbon sources with this
                      microorganism.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {583 - Innovative Synergisms (POF3-583)},
      pid          = {G:(DE-HGF)POF3-583},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000387984600006},
      pubmed       = {pmid:27288926},
      doi          = {10.1016/j.ymben.2016.06.003},
      url          = {https://juser.fz-juelich.de/record/811522},
}