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@ARTICLE{Jin:811561,
      author       = {Jin, Lei and Lange, Wienke and Kempmann, Annika and
                      Maybeck, Vanessa and Guenther, Anne and Gruteser, Nadine and
                      Baumann, A. and Offenhäusser, Andreas},
      title        = {{H}igh-efficiency transduction and specific expression of
                      {C}h{R}2opt for optogenetic manipulation of primary cortical
                      neurons mediated by recombinant adeno-associated viruses.},
      journal      = {Journal of biotechnology},
      volume       = {233},
      issn         = {0168-1656},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2016-04005},
      pages        = {171 - 180},
      year         = {2016},
      abstract     = {In recent years, optogenetic approaches have significantly
                      advanced the experimental repertoire of cellular and
                      functional neuroscience. Yet, precise and reliable methods
                      for specific expression of optogenetic tools remain
                      challenging. In this work, we studied the transduction
                      efficiency of seven different adeno-associated virus (AAV)
                      serotypes in primary cortical neurons and revealed
                      recombinant (r) AAV6 to be the most efficient for constructs
                      under control of the cytomegalovirus (CMV) promoter. To
                      further specify expression of the transgene, we exchanged
                      the CMV promoter for the human synapsin (hSyn) promoter. In
                      primary cortical-glial mixed cultures transduced with hSyn
                      promoter-containing rAAVs, expression of ChR2opt (a
                      Channelrhodopsin2 variant) was limited to neurons. In these
                      neurons action potentials could be reliably elicited upon
                      laser stimulation (473nm). The use of rAAV serotype alone to
                      restrict expression to neurons results in a lower
                      transduction efficiency than the use of a broader
                      transducing serotype with specificity conferred via a
                      restrictive promoter. Cells transduced with the hSyn driven
                      gene expression were able to elicit action potentials with
                      more spatially and temporally accurate illumination than
                      neurons electrofected with the CMV driven construct. The
                      hSyn promoter is particularly suited to use in AAVs due to
                      its small size. These results demonstrate that rAAVs are
                      versatile tools to mediate specific and efficient
                      transduction as well as functional and stable expression of
                      transgenes in primary cortical neurons.},
      cin          = {ICS-4 / ICS-8},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)ICS-8-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27416794},
      UT           = {WOS:000380819300019},
      doi          = {10.1016/j.jbiotec.2016.07.001},
      url          = {https://juser.fz-juelich.de/record/811561},
}