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000811935 1001_ $$0P:(DE-HGF)0$$aWojtowicz, H.$$b0
000811935 245__ $$aStructural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
000811935 260__ $$aLondon$$bPortland Press79505$$c2016
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000811935 520__ $$aBacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70–90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners.
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000811935 7001_ $$0P:(DE-HGF)0$$aProchnicka-Chalufour, A.$$b1
000811935 7001_ $$0P:(DE-HGF)0$$ade Amorim, G. C.$$b2
000811935 7001_ $$0P:(DE-HGF)0$$aRoudenko, O.$$b3
000811935 7001_ $$0P:(DE-HGF)0$$aSimenel, C.$$b4
000811935 7001_ $$0P:(DE-HGF)0$$aMalki, I.$$b5
000811935 7001_ $$0P:(DE-HGF)0$$aPehau-Arnaudet, G.$$b6
000811935 7001_ $$0P:(DE-HGF)0$$aGubellini, F.$$b7
000811935 7001_ $$0P:(DE-Juel1)158075$$aKoutsioumpas, Alexandros$$b8
000811935 7001_ $$0P:(DE-HGF)0$$aPerez, J.$$b9
000811935 7001_ $$0P:(DE-HGF)0$$aDelepelaire, P.$$b10
000811935 7001_ $$0P:(DE-HGF)0$$aDelepierre, M.$$b11
000811935 7001_ $$0P:(DE-HGF)0$$aFronzes, R.$$b12
000811935 7001_ $$0P:(DE-HGF)0$$aIzadi-Pruneyre, N.$$b13$$eCorresponding author
000811935 773__ $$0PERI:(DE-600)1473095-9$$a10.1042/BCJ20160131$$gVol. 473, no. 14, p. 2239 - 2248$$n14$$p2239 - 2248$$tBiochemical journal$$v473$$x1470-8728$$y2016
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