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@ARTICLE{Monzel:811940,
author = {Monzel, Cornelia and Schmidt, Daniel and Seifert, Udo and
Smith, Ana-Sunčana and Merkel, Rudolf and Sengupta, Kheya},
title = {{N}anometric thermal fluctuations of weakly confined
biomembranes measured with microsecond time-resolution},
journal = {Soft matter},
volume = {12},
number = {21},
issn = {1744-6848},
address = {London},
publisher = {Royal Soc. of Chemistry},
reportid = {FZJ-2016-04250},
pages = {4755 - 4768},
year = {2016},
abstract = {We probe the bending fluctuations of bio-membranes using
highly deflated giant unilamellar vesicles (GUVs) bound to a
substrate by a weak potential arising from generic
interactions. The substrate is either homogeneous, with GUVs
bound only by the weak potential, or is chemically
functionalized with a micro-pattern of very strong specific
binders. In both cases, the weakly adhered membrane is seen
to be confined at a well-defined distance above the surface
while it continues to fluctuate strongly. We quantify the
fluctuations of the weakly confined membrane at the
substrate proximal surface as well as of the free membrane
at the distal surface of the same GUV. This strategy enables
us to probe in detail the damping of fluctuations in the
presence of the substrate, and to independently measure the
membrane tension and the strength of the generic interaction
potential. Measurements were done using two complementary
techniques – dynamic optical displacement spectroscopy
(DODS, resolution: 20 nm, 10 μs), and dual wavelength
reflection interference contrast microscopy (DW-RICM,
resolution: 4 nm, 50 ms). After accounting for the
spatio-temporal resolution of the techniques, an excellent
agreement between the two measurements was obtained. For
both weakly confined systems we explore in detail the link
between fluctuations on the one hand and membrane tension
and the interaction potential on the other hand.},
cin = {ICS-7},
ddc = {530},
cid = {I:(DE-Juel1)ICS-7-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000379676800009},
pubmed = {pmid:27142463},
doi = {10.1039/C6SM00412A},
url = {https://juser.fz-juelich.de/record/811940},
}