%0 Journal Article
%A Chaves, Gustavo
%A Derst, Christian
%A Franzen, Arne
%A Mashimo, Yuta
%A Machida, Ryuichiro
%A Musset, Boris
%T Identification of an H$_{V}$1 voltage-gated proton channel in insects
%J The FEBS journal
%V 283
%N 8
%@ 1742-464X
%C Oxford [u.a.]
%I Wiley-Blackwell
%M FZJ-2016-04390
%P 1453 - 1464
%D 2016
%X The voltage-gated proton channel 1 (HV 1) is an important component of the cellular proton extrusion machinery and is essential for charge compensation during the respiratory burst of phagocytes. HV 1 has been identified in a wide range of eukaryotes throughout the animal kingdom, with the exception of insects. Therefore, it has been proposed that insects do not possess an HV 1 channel. In the present study, we report the existence of an HV 1-type proton channel in insects. We searched insect transcriptome shotgun assembly (TSA) sequence databases and found putative HV 1 orthologues in various polyneopteran insects. To confirm that these putative HV 1 orthologues were functional channels, we studied the HV 1 channel of Nicoletia phytophila (NpHV 1), an insect of the Zygentoma order, in more detail. NpHV 1 comprises 239 amino acids and is 33% identical to the human voltage-gated proton channel 1. Patch clamp measurements in a heterologous expression system showed proton selectivity, as well as pH- and voltage-dependent gating. Interestingly, NpHV 1 shows slightly enhanced pH-dependent gating compared to the human channel. Mutations in the first transmembrane segment at position 66 (Asp66), the presumed selectivity filter, lead to a loss of proton-selective conduction, confirming the importance of this aspartate residue in voltage-gated proton channels.Nucleotide sequence data have been deposited in the GenBank database under accession number KT780722.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26866814
%U <Go to ISI:>//WOS:000374680500007
%R 10.1111/febs.13680
%U https://juser.fz-juelich.de/record/817747