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000817747 1001_ $$0P:(DE-Juel1)162433$$aChaves, Gustavo$$b0$$ufzj
000817747 245__ $$aIdentification of an H$_{V}$1 voltage-gated proton channel in insects
000817747 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2016
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000817747 520__ $$aThe voltage-gated proton channel 1 (HV 1) is an important component of the cellular proton extrusion machinery and is essential for charge compensation during the respiratory burst of phagocytes. HV 1 has been identified in a wide range of eukaryotes throughout the animal kingdom, with the exception of insects. Therefore, it has been proposed that insects do not possess an HV 1 channel. In the present study, we report the existence of an HV 1-type proton channel in insects. We searched insect transcriptome shotgun assembly (TSA) sequence databases and found putative HV 1 orthologues in various polyneopteran insects. To confirm that these putative HV 1 orthologues were functional channels, we studied the HV 1 channel of Nicoletia phytophila (NpHV 1), an insect of the Zygentoma order, in more detail. NpHV 1 comprises 239 amino acids and is 33% identical to the human voltage-gated proton channel 1. Patch clamp measurements in a heterologous expression system showed proton selectivity, as well as pH- and voltage-dependent gating. Interestingly, NpHV 1 shows slightly enhanced pH-dependent gating compared to the human channel. Mutations in the first transmembrane segment at position 66 (Asp66), the presumed selectivity filter, lead to a loss of proton-selective conduction, confirming the importance of this aspartate residue in voltage-gated proton channels.Nucleotide sequence data have been deposited in the GenBank database under accession number KT780722.
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000817747 7001_ $$0P:(DE-HGF)0$$aDerst, Christian$$b1
000817747 7001_ $$0P:(DE-Juel1)131923$$aFranzen, Arne$$b2$$ufzj
000817747 7001_ $$0P:(DE-HGF)0$$aMashimo, Yuta$$b3
000817747 7001_ $$0P:(DE-HGF)0$$aMachida, Ryuichiro$$b4
000817747 7001_ $$0P:(DE-Juel1)159433$$aMusset, Boris$$b5$$eCorresponding author
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