TY  - JOUR
AU  - Chaves, Gustavo
AU  - Derst, Christian
AU  - Franzen, Arne
AU  - Mashimo, Yuta
AU  - Machida, Ryuichiro
AU  - Musset, Boris
TI  - Identification of an H$_{V}$1 voltage-gated proton channel in insects
JO  - The FEBS journal
VL  - 283
IS  - 8
SN  - 1742-464X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - FZJ-2016-04390
SP  - 1453 - 1464
PY  - 2016
AB  - The voltage-gated proton channel 1 (HV 1) is an important component of the cellular proton extrusion machinery and is essential for charge compensation during the respiratory burst of phagocytes. HV 1 has been identified in a wide range of eukaryotes throughout the animal kingdom, with the exception of insects. Therefore, it has been proposed that insects do not possess an HV 1 channel. In the present study, we report the existence of an HV 1-type proton channel in insects. We searched insect transcriptome shotgun assembly (TSA) sequence databases and found putative HV 1 orthologues in various polyneopteran insects. To confirm that these putative HV 1 orthologues were functional channels, we studied the HV 1 channel of Nicoletia phytophila (NpHV 1), an insect of the Zygentoma order, in more detail. NpHV 1 comprises 239 amino acids and is 33% identical to the human voltage-gated proton channel 1. Patch clamp measurements in a heterologous expression system showed proton selectivity, as well as pH- and voltage-dependent gating. Interestingly, NpHV 1 shows slightly enhanced pH-dependent gating compared to the human channel. Mutations in the first transmembrane segment at position 66 (Asp66), the presumed selectivity filter, lead to a loss of proton-selective conduction, confirming the importance of this aspartate residue in voltage-gated proton channels.Nucleotide sequence data have been deposited in the GenBank database under accession number KT780722.
LB  - PUB:(DE-HGF)16
C6  - pmid:26866814
UR  - <Go to ISI:>//WOS:000374680500007
DO  - DOI:10.1111/febs.13680
UR  - https://juser.fz-juelich.de/record/817747
ER  -