Home > Publications database > Identification of an H$_{V}$1 voltage-gated proton channel in insects > print

001     817747
005     20210129224020.0
024 7 _ |a 10.1111/febs.13680
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024 7 _ |a 1432-1033
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024 7 _ |a 1742-464X
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041 _ _ |a English
082 _ _ |a 540
100 1 _ |a Chaves, Gustavo
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245 _ _ |a Identification of an H$_{V}$1 voltage-gated proton channel in insects
260 _ _ |a Oxford [u.a.]
|c 2016
|b Wiley-Blackwell
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520 _ _ |a The voltage-gated proton channel 1 (HV 1) is an important component of the cellular proton extrusion machinery and is essential for charge compensation during the respiratory burst of phagocytes. HV 1 has been identified in a wide range of eukaryotes throughout the animal kingdom, with the exception of insects. Therefore, it has been proposed that insects do not possess an HV 1 channel. In the present study, we report the existence of an HV 1-type proton channel in insects. We searched insect transcriptome shotgun assembly (TSA) sequence databases and found putative HV 1 orthologues in various polyneopteran insects. To confirm that these putative HV 1 orthologues were functional channels, we studied the HV 1 channel of Nicoletia phytophila (NpHV 1), an insect of the Zygentoma order, in more detail. NpHV 1 comprises 239 amino acids and is 33% identical to the human voltage-gated proton channel 1. Patch clamp measurements in a heterologous expression system showed proton selectivity, as well as pH- and voltage-dependent gating. Interestingly, NpHV 1 shows slightly enhanced pH-dependent gating compared to the human channel. Mutations in the first transmembrane segment at position 66 (Asp66), the presumed selectivity filter, lead to a loss of proton-selective conduction, confirming the importance of this aspartate residue in voltage-gated proton channels.Nucleotide sequence data have been deposited in the GenBank database under accession number KT780722.
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700 1 _ |a Derst, Christian
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700 1 _ |a Franzen, Arne
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700 1 _ |a Mashimo, Yuta
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700 1 _ |a Machida, Ryuichiro
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700 1 _ |a Musset, Boris
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773 _ _ |a 10.1111/febs.13680
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