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@ARTICLE{Wasson:817878,
      author       = {Wasson, Anton and Bischof, Leanne and Zwart, Alec and Watt,
                      Michelle},
      title        = {{A} portable fluorescence spectroscopy imaging system for
                      automated root phenotyping in soil cores in the field},
      journal      = {The journal of experimental botany},
      volume       = {67},
      number       = {4},
      issn         = {1460-2431},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {FZJ-2016-04484},
      pages        = {1033 - 1043},
      year         = {2016},
      abstract     = {Root architecture traits are a target for pre-breeders.
                      Incorporation of root architecture traits into new cultivars
                      requires phenotyping. It is attractive to rapidly and
                      directly phenotype root architecture in the field, avoiding
                      laboratory studies that may not translate to the field. A
                      combination of soil coring with a hydraulic push press and
                      manual core-break counting can directly phenotype root
                      architecture traits of depth and distribution in the field
                      through to grain development, but large teams of people are
                      required and labour costs are high with this method. We
                      developed a portable fluorescence imaging system (BlueBox)
                      to automate root counting in soil cores with image analysis
                      software directly in the field. The lighting system was
                      optimized to produce high-contrast images of roots emerging
                      from soil cores. The correlation of the measurements with
                      the root length density of the soil cores exceeded the
                      correlation achieved by human operator measurements (R
                      2=0.68 versus 0.57, respectively). A BlueBox-equipped team
                      processed 4.3 cores/hour/person, compared with 3.7
                      cores/hour/person for the manual method. The portable,
                      automated in-field root architecture phenotyping system was
                      $16\%$ more labour efficient, $19\%$ more accurate, and
                      $12\%$ cheaper than manual conventional coring, and presents
                      an opportunity to directly phenotype root architecture in
                      the field as part of pre-breeding programs. The platform has
                      wide possibilities to capture more information about root
                      health and other root traits in the field.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000371020400005},
      pubmed       = {pmid:26826219},
      doi          = {10.1093/jxb/erv570},
      url          = {https://juser.fz-juelich.de/record/817878},
}