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@ARTICLE{Cookson:817935,
author = {Cookson, Sarah Jane and Yadav, Umesh Prasad and Klie,
Sebastian and Morcuende, Rosa and Usadel, Björn and Lunn,
John Edward and Stitt, Mark},
title = {{T}emporal kinetics of the transcriptional response to
carbon depletion and sucrose readdition in {A}rabidopsis
seedlings},
journal = {Plant, cell $\&$ environment},
volume = {39},
number = {4},
issn = {0140-7791},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2016-04527},
pages = {768 - 786},
year = {2016},
abstract = {To investigate whether the transcriptional response to
carbon (C) depletion and sucrose resupply depends on the
duration and severity of the C depletion, Arabidopsis
seedlings were grown in liquid culture and harvested 3, 6,
12, 24, 48 and 72 h after removing sucrose from the medium
and 30 min after resupplying sucrose at each time.
Expression profiling revealed early transcriptional
inhibition of cell wall synthesis and remodelling of
signalling, followed by induction of C recycling and
photosynthesis and general inhibition of growth. The
temporal sequence differed from the published response to
progressive exhaustion of C during a night and extended
night in vegetatively growing plants. The response to
sucrose readdition was conserved across the C-depletion time
course. Intriguingly, the vast majority of rapidly
responding transcripts decreased rather than increased. The
majority of transcripts that respond rapidly to sucrose and
many transcripts that respond during C depletion also
decrease after treating seedlings with the transcriptional
inhibitor cordycepin A. Comparison with published responses
to overexpression of otsA, AKIN10 and bZIP11 revealed that
many genes that respond to C depletion, and especially
sucrose resupply, respond to one or more of these
C-signalling components. Thus, multiple factors contribute
to C responsiveness, including many signalling components,
transcriptional regulation and transcript turnover.},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:26386165},
UT = {WOS:000373458800005},
doi = {10.1111/pce.12642},
url = {https://juser.fz-juelich.de/record/817935},
}