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000817936 1001_ $$0P:(DE-HGF)0$$aMacKellar, Drew$$b0
000817936 245__ $$aStreptomyces thermoautotrophicus does not fix nitrogen.
000817936 260__ $$aLondon$$bNature Publishing Group$$c2016
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000817936 520__ $$aStreptomyces thermoautotrophicus UBT1 has been described as a moderately thermophilic chemolithoautotroph with a novel nitrogenase enzyme that is oxygen-insensitive. We have cultured the UBT1 strain, and have isolated two new strains (H1 and P1-2) of very similar phenotypic and genetic characters. These strains show minimal growth on ammonium-free media, and fail to incorporate isotopically labeled N2 gas into biomass in multiple independent assays. The sdn genes previously published as the putative nitrogenase of S. thermoautotrophicus have little similarity to anything found in draft genome sequences, published here, for strains H1 and UBT1, but share >99% nucleotide identity with genes from Hydrogenibacillus schlegelii, a draft genome for which is also presented here. H. schlegelii similarly lacks nitrogenase genes and is a non-diazotroph. We propose reclassification of the species containing strains UBT1, H1, and P1-2 as a non-Streptomycete, non-diazotrophic, facultative chemolithoautotroph and conclude that the existence of the previously proposed oxygen-tolerant nitrogenase is extremely unlikely.
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000817936 7001_ $$0P:(DE-HGF)0$$aLieber, Lucas$$b1
000817936 7001_ $$0P:(DE-HGF)0$$aNorman, Jeffrey S$$b2
000817936 7001_ $$0P:(DE-HGF)0$$aBolger, Anthony$$b3
000817936 7001_ $$0P:(DE-HGF)0$$aTobin, Cory$$b4
000817936 7001_ $$0P:(DE-HGF)0$$aMurray, James W$$b5
000817936 7001_ $$0P:(DE-HGF)0$$aOksaksin, Mehtap$$b6
000817936 7001_ $$0P:(DE-HGF)0$$aChang, Roger L$$b7
000817936 7001_ $$0P:(DE-HGF)0$$aFord, Tyler J$$b8
000817936 7001_ $$0P:(DE-HGF)0$$aNguyen, Peter Q$$b9
000817936 7001_ $$0P:(DE-HGF)0$$aWoodward, Jimmy$$b10
000817936 7001_ $$0P:(DE-HGF)0$$aPermingeat, Hugo R$$b11
000817936 7001_ $$0P:(DE-HGF)0$$aJoshi, Neel S$$b12
000817936 7001_ $$0P:(DE-HGF)0$$aSilver, Pamela A$$b13
000817936 7001_ $$0P:(DE-Juel1)145719$$aUsadel, Björn$$b14$$ufzj
000817936 7001_ $$0P:(DE-HGF)0$$aRutherford, Alfred W$$b15
000817936 7001_ $$0P:(DE-HGF)0$$aFriesen, Maren L$$b16
000817936 7001_ $$0P:(DE-HGF)0$$aPrell, Jürgen$$b17$$eCorresponding author
000817936 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/srep20086$$gVol. 6, p. 20086 -$$p20086 -$$tScientific reports$$v6$$x2045-2322$$y2016
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