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@ARTICLE{MacKellar:817936,
      author       = {MacKellar, Drew and Lieber, Lucas and Norman, Jeffrey S and
                      Bolger, Anthony and Tobin, Cory and Murray, James W and
                      Oksaksin, Mehtap and Chang, Roger L and Ford, Tyler J and
                      Nguyen, Peter Q and Woodward, Jimmy and Permingeat, Hugo R
                      and Joshi, Neel S and Silver, Pamela A and Usadel, Björn
                      and Rutherford, Alfred W and Friesen, Maren L and Prell,
                      Jürgen},
      title        = {{S}treptomyces thermoautotrophicus does not fix nitrogen.},
      journal      = {Scientific reports},
      volume       = {6},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2016-04528},
      pages        = {20086 -},
      year         = {2016},
      abstract     = {Streptomyces thermoautotrophicus UBT1 has been described as
                      a moderately thermophilic chemolithoautotroph with a novel
                      nitrogenase enzyme that is oxygen-insensitive. We have
                      cultured the UBT1 strain, and have isolated two new strains
                      (H1 and P1-2) of very similar phenotypic and genetic
                      characters. These strains show minimal growth on
                      ammonium-free media, and fail to incorporate isotopically
                      labeled N2 gas into biomass in multiple independent assays.
                      The sdn genes previously published as the putative
                      nitrogenase of S. thermoautotrophicus have little similarity
                      to anything found in draft genome sequences, published here,
                      for strains H1 and UBT1, but share $>99\%$ nucleotide
                      identity with genes from Hydrogenibacillus schlegelii, a
                      draft genome for which is also presented here. H. schlegelii
                      similarly lacks nitrogenase genes and is a non-diazotroph.
                      We propose reclassification of the species containing
                      strains UBT1, H1, and P1-2 as a non-Streptomycete,
                      non-diazotrophic, facultative chemolithoautotroph and
                      conclude that the existence of the previously proposed
                      oxygen-tolerant nitrogenase is extremely unlikely.},
      cin          = {IBG-2},
      ddc          = {000},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582) / 583 - Innovative
                      Synergisms (POF3-583)},
      pid          = {G:(DE-HGF)POF3-582 / G:(DE-HGF)POF3-583},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:26833023},
      pmc          = {pmc:PMC4735515},
      UT           = {WOS:000368994900001},
      doi          = {10.1038/srep20086},
      url          = {https://juser.fz-juelich.de/record/817936},
}