000818233 001__ 818233
000818233 005__ 20210129224120.0
000818233 0247_ $$2Handle$$a2128/16233
000818233 037__ $$aFZJ-2016-04716
000818233 041__ $$aEnglish
000818233 1001_ $$0P:(DE-Juel1)133468$$aDahmen, Volker$$b0$$eCorresponding author$$ufzj
000818233 1112_ $$a19th Annual Meeting of the Society for Biological Radiation Research$$cErlangen$$d2016-09-26 - 2016-09-28$$gGBS 2016$$wGermany
000818233 245__ $$aSpecificity, damaging potential and cell killing of I-125 labeled Triplex-forming oligonucleotides in a transgenic cell line
000818233 260__ $$c2016
000818233 3367_ $$033$$2EndNote$$aConference Paper
000818233 3367_ $$2BibTeX$$aINPROCEEDINGS
000818233 3367_ $$2DRIVER$$aconferenceObject
000818233 3367_ $$2ORCID$$aCONFERENCE_POSTER
000818233 3367_ $$2DataCite$$aOutput Types/Conference Poster
000818233 3367_ $$0PUB:(DE-HGF)24$$2PUB:(DE-HGF)$$aPoster$$bposter$$mposter$$s1513256964_28638$$xAfter Call
000818233 520__ $$aIntroduction: Triplex-forming oligonucleotides (TFOs) are known for their ability to bind DNA in a sequence specific manner and are therefore a promising tool to manipulate genes or gene regulatory units in a cellular environment. Auger-Electron-Emitter-labeled TFOs can induce complex but localized damage to the DNA. Using radionuclide-labeled TFO the specificity of the target binding and the subsequent damage in the cellular environment were analysed by comparing the effects in two cell lines of which only one possessed the proper TFO target sequence.  Methods: The human carcinoma cell line SCL-II wildtype (WT) was stably transfected with a vector system (p2RT) producing a transgenic strain, SCL-II p2RT, containing the TFO-p2RT target sequence. TFO labeling with I-125 or P-32 was performed using the primer extension method. Efficient delivery of labeled TFO into SCL-II p2RT as well as SCL-II WT cells was ensured by electroporation with the Nucleofector I system (Lonza GmbH, Basel). Subsequently, specific target binding was visualized by restriction digest of isolated genomic DNA and autoradiographic analysis. DNA damage and survival rate were determined with the 53BP1-foci and the colony forming assay (CFA).Results: Autoradiographic analysis revealed a specific triplex formation at the p2RT vector target sequence, confirming the specific binding. However, the 53BP1-foci assay and the CFA detected a similar increased level of DNA damage and a pronounced reduction in cell survival in both cell strains per cumulated decays. Thus, the different target status in the two cell strains was not reflected on damage level and in survival rate. Conclusions: Even though a high degree of specific TFO-p2RT binding to its target site could be verified, the 53BP1 and CFA data indicate that also a considerable amount of alternative DNA damaging is occurring.
000818233 536__ $$0G:(DE-HGF)POF3-899$$a899 - ohne Topic (POF3-899)$$cPOF3-899$$fPOF III$$x0
000818233 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000818233 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b1$$ufzj
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.pdf$$yOpenAccess
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.gif?subformat=icon$$xicon$$yOpenAccess
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000818233 8564_ $$uhttps://juser.fz-juelich.de/record/818233/files/Abstract_ERRS-GBS_Essen_2017.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000818233 909CO $$ooai:juser.fz-juelich.de:818233$$pdriver$$pVDB$$popen_access$$popenaire
000818233 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133468$$aForschungszentrum Jülich$$b0$$kFZJ
000818233 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133469$$aForschungszentrum Jülich$$b1$$kFZJ
000818233 9131_ $$0G:(DE-HGF)POF3-899$$1G:(DE-HGF)POF3-890$$2G:(DE-HGF)POF3-800$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000818233 9141_ $$y2017
000818233 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000818233 920__ $$lyes
000818233 9201_ $$0I:(DE-Juel1)S-US-20090406$$kS-US$$lSicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie$$x0
000818233 980__ $$aposter
000818233 980__ $$aVDB
000818233 980__ $$aUNRESTRICTED
000818233 980__ $$aI:(DE-Juel1)S-US-20090406
000818233 9801_ $$aFullTexts