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@ARTICLE{Zhu:818252,
author = {Zhu, Geng and Du, Liping and Jin, Lei and Offenhäusser,
Andreas},
title = {{E}ffects of {M}orphology {C}onstraint on
{E}lectrophysiological {P}roperties of {C}ortical {N}eurons},
journal = {Scientific reports},
volume = {6},
issn = {2045-2322},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2016-04730},
pages = {23086},
year = {2016},
abstract = {There is growing interest in engineering nerve cells in
vitro to control architecture and connectivity of cultured
neuronal networks or to build neuronal networks with
predictable computational function. Pattern technologies,
such as micro-contact printing, have been developed to
design ordered neuronal networks. However,
electrophysiological characteristics of the single patterned
neuron haven’t been reported. Here, micro-contact
printing, using polyolefine polymer (POP) stamps with high
resolution, was employed to grow cortical neurons in a
designed structure. The results demonstrated that the
morphology of patterned neurons was well constrained, and
the number of dendrites was decreased to be about 2. Our
electrophysiological results showed that alterations of
dendritic morphology affected firing patterns of neurons and
neural excitability. When stimulated by current, though both
patterned and un-patterned neurons presented regular
spiking, the dynamics and strength of the response were
different. The un-patterned neurons exhibited a
monotonically increasing firing frequency in response to
injected current, while the patterned neurons first
exhibited frequency increase and then a slow decrease. Our
findings indicate that the decrease in dendritic complexity
of cortical neurons will influence their
electrophysiological characteristics and alter their
information processing activity, which could be considered
when designing neuronal circuitries.},
cin = {ICS-8},
ddc = {000},
cid = {I:(DE-Juel1)ICS-8-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000373566700001},
doi = {10.1038/srep23086},
url = {https://juser.fz-juelich.de/record/818252},
}