000819304 001__ 819304
000819304 005__ 20240619091211.0
000819304 0247_ $$2doi$$a10.3389/fbioe.2016.00046
000819304 0247_ $$2Handle$$a2128/12984
000819304 0247_ $$2pmid$$apmid:27379230
000819304 0247_ $$2WOS$$aWOS:000390385500001
000819304 0247_ $$2altmetric$$aaltmetric:8751161
000819304 037__ $$aFZJ-2016-05005
000819304 041__ $$aEnglish
000819304 082__ $$a570
000819304 1001_ $$0P:(DE-Juel1)156416$$aAlbers, Jonas$$b0
000819304 245__ $$aSignal Propagation between Neuronal Populations Controlled by Micropatterning
000819304 260__ $$aLausanne$$bFrontiers Media$$c2016
000819304 3367_ $$2DRIVER$$aarticle
000819304 3367_ $$2DataCite$$aOutput Types/Journal article
000819304 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1479911802_25248
000819304 3367_ $$2BibTeX$$aARTICLE
000819304 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000819304 3367_ $$00$$2EndNote$$aJournal Article
000819304 520__ $$aThe central nervous system consists of an unfathomable number of functional networks enabling highly sophisticated information processing. Guided neuronal growth with a well-defined connectivity and accompanying polarity is essential for the formation of these networks. To investigate how two-dimensional protein patterns influence neuronal outgrowth with respect to connectivity and functional polarity between adjacent populations of neurons, a microstructured model system was established. Exclusive cell growth on patterned substrates was achieved by transferring a mixture of poly-l-lysine and laminin to a cell-repellent glass surface by microcontact printing. Triangular structures with different opening angle, height, and width were chosen as a pattern to achieve network formation with defined behavior at the junction of adjacent structures. These patterns were populated with dissociated primary cortical embryonic rat neurons and investigated with respect to their impact on neuronal outgrowth by immunofluorescence analysis, as well as their functional connectivity by calcium imaging. Here, we present a highly reproducible technique to devise neuronal networks in vitro with a predefined connectivity induced by the design of the gateway. Daisy-chained neuronal networks with predefined connectivity and functional polarity were produced using the presented micropatterning method. Controlling the direction of signal propagation among populations of neurons provides insights to network communication and offers the chance to investigate more about learning processes in networks by external manipulation of cells and signal cascades.
000819304 536__ $$0G:(DE-HGF)POF3-552$$a552 - Engineering Cell Function (POF3-552)$$cPOF3-552$$fPOF III$$x0
000819304 588__ $$aDataset connected to CrossRef
000819304 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b1$$eCorresponding author$$ufzj
000819304 773__ $$0PERI:(DE-600)2719493-0$$a10.3389/fbioe.2016.00046$$gVol. 4$$p46$$tFrontiers in Bioengineering and Biotechnology$$v4$$x2296-4185$$y2016
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.pdf$$yOpenAccess
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.gif?subformat=icon$$xicon$$yOpenAccess
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000819304 8564_ $$uhttps://juser.fz-juelich.de/record/819304/files/fbioe-04-00046.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000819304 8767_ $$92016-07-25$$d2016-07-25$$eAPC$$jZahlung erfolgt$$lDeposit: Frontiers$$zUSD 1311,-
000819304 909CO $$ooai:juser.fz-juelich.de:819304$$pdnbdelivery$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire
000819304 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128713$$aForschungszentrum Jülich$$b1$$kFZJ
000819304 9131_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000819304 9141_ $$y2016
000819304 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000819304 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000819304 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000819304 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000819304 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000819304 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000819304 920__ $$lyes
000819304 9201_ $$0I:(DE-Juel1)ICS-8-20110106$$kICS-8$$lBioelektronik$$x0
000819304 9801_ $$aFullTexts
000819304 980__ $$ajournal
000819304 980__ $$aVDB
000819304 980__ $$aUNRESTRICTED
000819304 980__ $$aI:(DE-Juel1)ICS-8-20110106
000819304 980__ $$aAPC
000819304 981__ $$aI:(DE-Juel1)IBI-3-20200312