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@ARTICLE{Albers:819304,
      author       = {Albers, Jonas and Offenhäusser, Andreas},
      title        = {{S}ignal {P}ropagation between {N}euronal {P}opulations
                      {C}ontrolled by {M}icropatterning},
      journal      = {Frontiers in Bioengineering and Biotechnology},
      volume       = {4},
      issn         = {2296-4185},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2016-05005},
      pages        = {46},
      year         = {2016},
      abstract     = {The central nervous system consists of an unfathomable
                      number of functional networks enabling highly sophisticated
                      information processing. Guided neuronal growth with a
                      well-defined connectivity and accompanying polarity is
                      essential for the formation of these networks. To
                      investigate how two-dimensional protein patterns influence
                      neuronal outgrowth with respect to connectivity and
                      functional polarity between adjacent populations of neurons,
                      a microstructured model system was established. Exclusive
                      cell growth on patterned substrates was achieved by
                      transferring a mixture of poly-l-lysine and laminin to a
                      cell-repellent glass surface by microcontact printing.
                      Triangular structures with different opening angle, height,
                      and width were chosen as a pattern to achieve network
                      formation with defined behavior at the junction of adjacent
                      structures. These patterns were populated with dissociated
                      primary cortical embryonic rat neurons and investigated with
                      respect to their impact on neuronal outgrowth by
                      immunofluorescence analysis, as well as their functional
                      connectivity by calcium imaging. Here, we present a highly
                      reproducible technique to devise neuronal networks in vitro
                      with a predefined connectivity induced by the design of the
                      gateway. Daisy-chained neuronal networks with predefined
                      connectivity and functional polarity were produced using the
                      presented micropatterning method. Controlling the direction
                      of signal propagation among populations of neurons provides
                      insights to network communication and offers the chance to
                      investigate more about learning processes in networks by
                      external manipulation of cells and signal cascades.},
      cin          = {ICS-8},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27379230},
      UT           = {WOS:000390385500001},
      doi          = {10.3389/fbioe.2016.00046},
      url          = {https://juser.fz-juelich.de/record/819304},
}