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@ARTICLE{Jaeger:819312,
      author       = {Jaeger, Alexandra and Fröhlich, Michael and Klum, Susanne
                      and Lantow, Margareta and Viergutz, Torsten and Weiss,
                      Dieter G. and Kriehuber, Ralf},
      title        = {{C}haracterization of {A}poptosis {S}ignaling {C}ascades
                      {D}uring the {D}ifferentiation {P}rocess of {H}uman {N}eural
                      {R}e{N}cell {VM} {P}rogenitor {C}ells {I}n {V}itro},
      journal      = {Cellular and molecular neurobiology},
      volume       = {35},
      number       = {8},
      issn         = {1573-6830},
      address      = {Dordrecht},
      publisher    = {Springer Science + Business Media B.V},
      reportid     = {FZJ-2016-05013},
      pages        = {1203 - 1216},
      year         = {2015},
      abstract     = {Apoptosis is an essential physiological process
                      accompanying the development of the central nervous system
                      and human neurogenesis. However, the time scale and the
                      underlying molecular mechanisms are yet poorly understood.
                      Due to this fact, we investigated the functionality and
                      general inducibility of apoptosis in the human neural
                      ReNcell VM progenitor cell line during differentiation and
                      also after exposure to staurosporine (STS) and ultraviolet B
                      (UVB) irradiation. Transmission light microscopy, flow
                      cytometry, and Western-/Immunoblot analysis were performed
                      to compare proliferating and differentiating, in addition to
                      STS- and UVB-treated cells. In particular, from 24 to 72 h
                      post-initiation of differentiation, G0/G1 cell cycle arrest,
                      increased loss of apoptotic cells, activation of
                      pro-apoptotic BAX, Caspase-3, and cleavage of its substrate
                      PARP were observed during cell differentiation and, to a
                      higher extent, after treatment with STS and UVB. We conclude
                      that redundant or defective cells are eliminated by
                      apoptosis, while otherwise fully differentiated cells were
                      less responsive to apoptosis induction by STS than
                      proliferating cells, likely as a result of reduced APAF-1
                      expression, and increased levels of BCL-2. These data
                      provide the evidence that apoptotic mechanisms in the neural
                      ReNcell VM progenitor cell line are not only functional, but
                      also inducible by external stimuli like growth factor
                      withdrawal or treatment with STS and UVB, which marks this
                      cell line as a suitable model to investigate apoptosis
                      signaling pathways in respect to the differentiation
                      processes of human neural progenitor cells in vitro.},
      cin          = {S-US},
      ddc          = {610},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000362882300013},
      doi          = {10.1007/s10571-015-0213-7},
      url          = {https://juser.fz-juelich.de/record/819312},
}