% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Hlsemann:819525,
      author       = {Hülsemann, Maren and Zafiu, Christian and Kühbach, Katja
                      and Lühmann, Nicole and Herrmann, Yvonne and Peters,
                      Luriano and Linnartz, Christina and Willbold, Johannes and
                      Kravchenko, Kateryna and Kulawik, Andreas and Willbold,
                      Sabine and Bannach, Oliver and Willbold, Dieter},
      title        = {{B}iofunctionalized {S}ilica {N}anoparticles: {S}tandards
                      in {A}myloid-β {O}ligomer-{B}ased {D}iagnosis of
                      {A}lzheimer’s {D}isease},
      journal      = {Journal of Alzheimer's disease},
      volume       = {54},
      number       = {1},
      issn         = {1875-8908},
      address      = {Amsterdam},
      publisher    = {IOS Press},
      reportid     = {FZJ-2016-05168},
      pages        = {79 - 88},
      year         = {2016},
      abstract     = {Amyloid-β (Aβ) oligomers represent a promising biomarker
                      for the early diagnosis of Alzheimer’s disease (AD).
                      However, state-of-the-art methods for immunodetection of Aβ
                      oligomers in body fluids show a large variability and lack a
                      reliable and stable standard that enables the reproducible
                      quantitation of Aβ oligomers. At present, the only
                      available standard applied in these assays is based on a
                      random aggregation process of synthetic Aβ and has neither
                      a defined size nor a known number of epitopes. In this
                      report, we generated a highly stable standard in the size
                      range of native Aβ oligomers that exposes a defined number
                      of epitopes. The standard consists of a silica nanoparticle
                      (SiNaP), which is functionalized with Aβ peptides on its
                      surface (Aβ-SiNaP). The different steps of Aβ-SiNaP
                      synthesis were followed by microscopic, spectroscopic and
                      biochemical analyses. To investigate the performance of
                      Aβ-SiNaPs as an appropriate standard in Aβ oligomer
                      immunodetection, Aβ-SiNaPs were diluted in cerebrospinal
                      fluid and quantified down to a concentration of 10 fM in
                      the sFIDA (surface-based fluorescence intensity distribution
                      analysis) assay. This detection limit corresponds to an Aβ
                      concentration of 1.9 ng l–1 and lies in the sensitivity
                      range of currently applied diagnostic tools based on Aβ
                      oligomer quantitation. Thus, we developed a highly stable
                      and well-characterized standard for the application in Aβ
                      oligomer immunodetection assays that finally allows the
                      reproducible quantitation of Aβ oligomers down to single
                      molecule level and provides a fundamental improvement for
                      the worldwide standardization process of diagnostic methods
                      in AD research.},
      cin          = {ICS-6 / ZEA-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-6-20110106 / I:(DE-Juel1)ZEA-3-20090406},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000383838400007},
      pubmed       = {pmid:27472876},
      doi          = {10.3233/JAD-160253},
      url          = {https://juser.fz-juelich.de/record/819525},
}