%0 Grant
%A Kreysing, Eva
%T Poster Award "Surface Plasmon Resonance Microscopy of the Cell-Chip Interface"
%C Germany
%I German Biophysics Society
%M FZJ-2016-05207
%D 2016
%X Poster: "Surface Plasmon Resonance Microscopy of the Cell-Chip Interface"Longterm investigation of neuronal networks require non-invasive recordings of the electrical signals. A good coupling between the biological and electronic system is crucial and depends particularly upon the  cell-chip  distance.  The  cell-chip  distance  is  an  important  parameter  towards  a  good  sealing,  with closer contact leading to a decreased signal dissipation in the cell-electrode cleft. We therefore try to optimize  the  contact  geometry  of  said  interface  using  protein  and  lipid  coatings.  In  order  to  measure the  distances  between  the  cell  membrane  and  the  chip  surface  in  vitro,  we  built  a  surface  plasmon resonance microscope (SPRM). With gold coated sapphire chips as the substrate for the cell culture, it is possible to excite plasmons (collective  electron  oscillations)  in  the  gold  layer  by  illuminating  it  under  a  specific  angle.  The resonance  frequency  of  the  plasmons  depends  strongly  upon  the  dielectric  constant  of  the  gold's environment.  In  turn  the  angle  spectrum  of  the  reflected  light  depends upon  said  resonance frequencies. Due to these dependencies it is possible to deduce the cell-substrate distance.Our microscope is capable of imaging the interface in two different modes. The field of view in the live imaging  mode  is  around  65  um  x  65  um.This  is  useful  for  determining  the  region  of  interest  for  the scanning  mode.  This  mode  uses  localized  surface  plasmons  to  measure  the  cell-substrate  distance. The resolution in z-direction lies in the nanometer range. This allows us to accurately characterize the cell-chip interface.Since SPRM is non-invasive and label free it is suited for longterm investigations.It is therefore possible to observe the development of neuronal networks over several week.
%F PUB:(DE-HGF)38
%9 Award
%U https://juser.fz-juelich.de/record/819577